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Fig. 2 | BMC Microbiology

Fig. 2

From: Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition

Fig. 2

Biochemical characterization of PIP-treated stationary phase cells. Cell cultures were treated with piperacillin (PIP-treated) or water (untreated) at t = 4 h. At t = 24 h, measurements of DNA (a), ATP, protein, and RNA content (b) were carried out. a Cell cultures were fixed and stained with PicoGreen for DNA content and chromosome number assessment using flow cytometry. Numbers above the brackets indicate chromosome copy number. The chromosome number scale was determined with stationary-phase cell cultures of known DNA content (Additional file 7: Figure S7). b Cells were pelleted for RNA extraction, sonicated for protein concentration determination by the Bradford assay, or diluted to an OD600 of ~ 0.1 for ATP measurements using the BacTiter-Glo assay. Abundances were calculated on a per cell basis, with cell number quantified by flow cytometry, and are presented relative to untreated. * p < 0.05 (t-test). Data represent three or more biological replicates. Each data point was denoted as mean ± s.e

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