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Fig. 1 | BMC Microbiology

Fig. 1

From: Detection of Candidatus Neoehrlichia mikurensis in Norway up to the northern limit of Ixodes ricinus distribution using a novel real time PCR test targeting the groEL gene

Fig. 1

Multiple sequence alignment of the PCR target regions of groEL in Ca. N. mikurensis (CNM), Ca. N. lotori and selected Ehrlichia species. Dots indicate identity to the reference sequence, AB084583; letters indicate differences; hyphens indicate gaps or no sequence. The target regions for the primers and probe are highlighted in yellow and green respectively. Mismatches within the primer/probe target regions that give stable G:T basepairs are highlighted in blue. Destabilising mismatches (variants resulting in A:C, purine:purine or pyrimidine:pyrimidine) are highlighted in red. For reasons of space, sequence accession numbers for Ca. N. lotoris, Ehrlichia ewingii, Candidatus E. shimanensis, two sequence variants of E. ruminantium and E. chafeensis have been omitted from the figure; these are: EF633745, AF195273, AB074462, AB625796, DQ647005 and JQ085941 respectively

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BMC Microbiology

ISSN: 1471-2180

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