Genetic characteristics and virulence of Listeria monocytogenes isolated from fresh vegetables in China

BMC Microbiology

Table 3 Primers used for virulence genes detection of Listeria monocytogenes strains

Genes	Sequences (5′-3′)	Tm (°C)	Length (bp)	Reference
prfA	CTGTTGGAGCTCTTCTTGGTGAAGCAATCG	60	1060	[35]
prfA	AGCAACCTCGGTACCATATACTAACTC	60	1060	[35]
mpl	ATAGCTTTTCAGGCTCATTTCA	60	1184	this study
mpl	ATAGCTTTTCAGGCTCATTTCA	60	1184	this study
plcA	CTGCTTGAGCGTTCATGTCTCATCCCCC	60	1484	[35]
plcA	CATGGGTTTCACTCTCCTTCTAC	60	1484	[35]
inlB	GATATTGTGCCACTTTCAGGT	60	367	[36]
inlB	CCTCTTTCAGTGGTTGGGT	60	367	[36]
plcA	CTGCTTGAGCGTTCATGTCTCATCCCCC	60	436	[37]
plcA	CATGGGTTTCACTCTCCTTCTAC	60	436	[37]
hly	CTTGCAACTGCTCTTTAGTAACAGC	60	706	[38]
hly	ACAAGCTGCACCTGTTGCAG	60	706	[38]
iap	ACAAGCTGCACCTGTTGCAG	60	131	[39]
iap	TGACAGCGTGTGTAGTAGCA	60	131	[39]
actA	CGCCGCGGAAATTAAAAAAAGA	60	839	[40]
actA	ACGAAGGAACCGGGCTGCTAG	60	839	[40]
llxS	TTATTGCATCAATTGTTCTAGGG	52	200	[41]
llxS	CCCCTATAAACATCATGCTAGTG	52	200	[41]
ptsA ^a	TCCTTTTTCTTTGTTGCGGA	52	450	[13]
ptsA ^a	TCTGAAGCTGTACGAAGACA	52	450	[13]

ISSN: 1471-2180