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Table 1 Bacterial strains and plasmids used in this study

From: Carbohydrate metabolic systems present on genomic islands are lost and gained in Vibrio parahaemolyticus

Bacterial strains

Genotype or description

References or sources

Vibrio parahaemolyticus

 RIMD2210633

O3:K6 clinical isolate, Smr

[8]

 Δeda1

RIMD2210633 Δeda1 (VP0065), Smr

This study

 UCM-V493

O2:K28 environmental isolate, Smr

[53]

 Δsglt

UCM-V493 Δsglt (VPUCM_0844), Smr

This study

 RIMDpSGLT

RIMD2210633 harboring pBBRsglt, Cmr

This study

Escherichia coli

 BW25113

Wild-Type E. coli K12 strain

[54]

 DH5αλpir

 

Laboratory collection

 B2155λpir

ΔdapA::emr pir, for bacterial conjugation

Laboratory collection

 Klebsilla pneumoniae K2

 

Laboratory collection

 Vibrio cholerae N16961

O1 El Tor, Smr

[55]

Plasmids

 pJet1.2/blunt

Cloning vector/Ampr

Fermentas

 Pjet1.2-edA1SOEAD

pJet1.2 harboring truncated eda1, Ampr

This study

 pDS132

Suicide plasmid, Cmr, SacB

[56]

 pDSΔeda1

pDS132 harboring truncated eda1, Cmr

This study

 Pjet1.2-sgltSOEAD

pJet1.2 harboring truncated sglt, Ampr

This study

 Pjet1.2-sglt

pJet1.2 harboring sglt, Ampr

This study

 pDSΔsglt

pDS132 harboring truncated sglt, Cmr

This study

 pBBR1MCS

Expression vector, lacZ promoter, Cmr

[57]

 pBBRsglt

pBBR1MCS harboring full-length sglt, Cmr

This study