Fig. 11

MRSA t127/4 and MRSA t223/20 biofilms treated with UPMK_1 and UPMK_2 for 2, 4, 6,8,12 and 24 h. A1 and B1 are biofilm biomass treated with the phages at MOI ̴ 1 (OD570 reading after CV staining). The estimated number of viable bacterial cells is shown in A2 and B2, while A3 and B3 represent phage particles in the biofilms treated with UPMK_1 for 6 h and with UPMK_2 for 8 h, respectively. The assays were performed severally to assess the ability of the phages to degrade the biofilm, and thrice to estimate the other values. The values are shown as the mean ± standard deviation of the values; statistical significance of biofilm reduction was reported at p < 0.05