Fig. 1

Examples of MRSA isolated phage plaques. a, b, c, d and e represent phage UPMK_1 infected bacterial lawn host MRSA t127/4, while f, g, h and i represent phage UPMK_2 infected bacterial lawn host MRSA t223/20. Using 0.6% agar overlay method plaques morphology of UPMK_1 (B and E) and UPMK_2 (G) appeared as lysis center surrounded by turbid halos. The phage lysate in plates C, D, H, I was applied in 10-fold serial dilutions to lawn of the host bacteria by spotting 10 μl from the phage dilution. The turbid halo around the lysis zone appeared clearer and its size increased as shown in D and I after longer incubation