Skip to main content

Table 3 Substrate utilization and metabolite production of co-cultures. Co-cultures were grown in YCFA supplied with glucose and glycerol (both 50 mM) at 37 °C for 24 h. Substrate utilization and metabolite production were quantified with HPLC-RI. n = number of biological replicates. Values are presented as mean ± standard deviation

From: Gut microbial beta-glucuronidase and glycerol/diol dehydratase activity contribute to dietary heterocyclic amine biotransformation

Co-culturea Substrate n Substrate utilization (mM) Metabolite production (mM)
Formate Acetate Propionate Butyrate 1,3-PD
F. prausnitzii and F. plautii glycerol
glucose
4 −11.2 ± 6.6A*
− 13.4 ± 4.4A
13.1 ± 3.3A −6.7 ± 3.5A − 1.5 ± 1.1A 13.3 ± 2.2A 1.2 ± 0.4A
F. prausnitzii and L. reuteri glycerol
glucose
3 −26.5 ± 0.6B
− 20.6 ± 3.0B
10.7 ± 1.9A −2.0 ± 4.9A −1.6 ± 0.5A 10.8 ± 1.8A 15.9 ± 3.6B
F. prausnitzii and B. obeum glycerol
glucose
2 − 1.8 ± 3.0B
− 23.0 ± 1.1C
11.5 ± 1.3A 14.4 ± 2.8B − 1.6 ± 0.4A 9.3 ± 2.8A 0A
  1. *Different letters represent the significant differences (p < 0.05) on the substrate utilization or metabolite production between different co-cultures using multiple comparisons with 2-way ANOVA