Gut microbial beta-glucuronidase and glycerol/diol dehydratase activity contribute to dietary heterocyclic amine biotransformation

Table 3 Substrate utilization and metabolite production of co-cultures. Co-cultures were grown in YCFA supplied with glucose and glycerol (both 50 mM) at 37 °C for 24 h. Substrate utilization and metabolite production were quantified with HPLC-RI. n = number of biological replicates. Values are presented as mean ± standard deviation

Co-culture^a	Substrate	n	Substrate utilization (mM)	Metabolite production (mM)
Co-culture^a	Substrate	n	Substrate utilization (mM)	Formate	Acetate	Propionate	Butyrate	1,3-PD
F. prausnitzii and F. plautii	glycerol glucose	4	−11.2 ± 6.6^A* − 13.4 ± 4.4^A	13.1 ± 3.3^A	−6.7 ± 3.5^A	− 1.5 ± 1.1^A	13.3 ± 2.2^A	1.2 ± 0.4^A
F. prausnitzii and L. reuteri	glycerol glucose	3	−26.5 ± 0.6^B − 20.6 ± 3.0^B	10.7 ± 1.9^A	−2.0 ± 4.9^A	−1.6 ± 0.5^A	10.8 ± 1.8^A	15.9 ± 3.6^B
F. prausnitzii and B. obeum	glycerol glucose	2	− 1.8 ± 3.0^B − 23.0 ± 1.1^C	11.5 ± 1.3^A	14.4 ± 2.8^B	− 1.6 ± 0.4^A	9.3 ± 2.8^A	0^A

*Different letters represent the significant differences (p < 0.05) on the substrate utilization or metabolite production between different co-cultures using multiple comparisons with 2-way ANOVA

ISSN: 1471-2180