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Table 2 Substrate utilization and metabolite production of single strains in the presence of glycerol or 1,2-propanediol. Strains were grown in anaerobic YCFA supplied with glycerol or 1,2-propanediol (1,2-PD) (both 50 mM) at 37 °C for 24 h. E. eligens did not grow and utilize glycerol and 1,2-PD. n.d. = not determined; n = number of biological replicates. Values are presented as mean ± standard deviation

From: Gut microbial beta-glucuronidase and glycerol/diol dehydratase activity contribute to dietary heterocyclic amine biotransformation

Straina Substrate n Substrate utilization (mM) Metabolite production (mM)
Formate Acetate Propionate Butyrate 1,3-PD
B. obeum 1,2-PD 4 −17.9 ± 4.3A* 0A 1.5 ± 1.7AB 5.2 ± 0.4A − 0.2 ± 0.6A n.d.
glycerol 6 −2.1 ± 1.2a 0a 1.5 ± 1.4a − 0.6 ± 0.4a 0a 0
E. hallii 1,2-PD 6 −46.6 ± 6.4B 1.0 ± 0.8A − 1.6 ± 7.8A 19.3 ± 8.0B 4.3 ± 0.7B n.d.
glycerol 6 − 13.3 ± 3.0b 0.7 ± 1.0b − 4.6 ± 2.5b 0 ± 0.8a 0 ± 0.8a 1.9 ± 0.7a
F. prausnitzii 1,2-PD 2 − 0.7C 0A −1.4 A 0C 1.5A n.d.
glycerol 3 −1.6 ± 0.4c 0.9 ± 1.6a 1.3 ± 0.5a 0.3 ± 0.2a 0.3 ± 0.6a 0 a
F. plautii 1,2-PD 4 −48.1 ± 1.2B 0A 2.2 ± 1.4A 11.6 ± 3.9B 4.1 ± 0.3B n.d.
glycerol 4 −9.1 ± 3.0c 0a 5.2 ± 0.7c −0.5 ± 0.4a 4.3 ± 0.5b 1.2 ± 0.7a
I. butyriciproduens 1,2-PD 5 −1.2 ± 2.9C 0A 2.6 ± 2.6A − 0.1 ± 0.5C 5.4 ± 0.6B n.d.
glycerol 3 − 0.8 ± 1.4ac 0a 2.5 ± 1.7ac −0.1 ± 0.1a 5.3 ± 0.5b 3.0 ± 0.1b
L. reuteri 1,2-PD 3 −9.8 ± 4.2C 0A −1.0 ± 1.1A 0.9 ± 0.5C 0A n.d.
glycerol 3 −14.4 ± 8.8b 0a −0.4 ± 1.1a 0a 0a 5.2 ± 0b
R. hominis 1,2-PD 3 0D 0A 0A 0C 0A n.g.
glycerol 5 −5.9 ± 1.1c 0a −8.5 ± 1.3d −0.5 ± 0.2a 7.4 ± 1.6b 0
R. intestinalis 1,2-PD 3 −0.4 ± 0.4C 0A − 0.4 ± 0.4A −0.3 ± 0.1C 0.4 ± 0.2A n.d.
glycerol 3 −1.4 ± 0.6ac 0a 1.3 ± 1.0a 0.4 ± 0.4a 0.4 ± 0.2a 0a
R. gnavus 1,2-PD 3 −18.0 ± 5.1A 1.1 ± 1.9A 2.2 ± 1.4AB 7.9 ± 2.4A 0.2 ± 0.3A n.d.
glycerol 3 − 1.4 ± 1.2a 0a 0.8 ± 1.4ae 0a 0a 0.2 ± 0.3a
V. dispar 1,2-PD 4 −1.7 ± 2.4D 1.4 ± 1.6A 0 ± 2.3AB 0.6 ± 0.8C 0A n.d.
glycerol 4 −4.4 ± 1.4ac 6.6 ± 1.2c −2.4 ± 1.9be − 0.5 ± 0.5a −0.2 ± 0.4a 0a
B. fragilis 1,2-PD 4 −1.0 ± 1.4C 0A −0.1 ± 1.6A 0.3 ± 0.5C −0.4 ± 0.4A n.d.
glycerol 3 − 1.0 ± 0.9ac 0a 0 ± 0.5a 0.6 ± 0.6a 0a 0a
C. freundii 1,2-PD 3 −1.4 ± 1.8D 5.4 ± 0.6B 7.2 ± 1.2B 1.5 ± 0.6D 0A n.d.
glycerol 3 −2.1 ± 3.2a 6.0 ± 0.5c 5.3 ± 2.0c −0.8 ± 0.8a 0a 0a
K. pneumoniae 1,2-PD 3 − 42.7 ± 1.1B 5.3 ± 0.1B 3.1 ± 1.5AB 16.7 ± 1.0B 0A n.d.
glycerol 3 − 48.7 ± 0.8d 17.5 ± 1.2d 10.7 ± 0.2f 0a 0a 16.4 ± 3.3c
  1. *Different letters represent the significant differences (p < 0.05) on the substrate utilization or metabolite production between different strains using multiple comparisons with 2-way ANOVA. Capital letters are for 1,2-PD and the corresponding metabolites, small letters are for glycerol and the corresponding metabolites