Table 2 Substrate utilization and metabolite production of single strains in the presence of glycerol or 1,2-propanediol. Strains were grown in anaerobic YCFA supplied with glycerol or 1,2-propanediol (1,2-PD) (both 50 mM) at 37 °C for 24 h. E. eligens did not grow and utilize glycerol and 1,2-PD. n.d. = not determined; n = number of biological replicates. Values are presented as mean ± standard deviation

B. obeum

1,2-PD

−17.9 ± 4.3^A*

0^A

1.5 ± 1.7^AB

5.2 ± 0.4^A

− 0.2 ± 0.6^A

n.d.

glycerol

−2.1 ± 1.2^a

0^a

1.5 ± 1.4^a

− 0.6 ± 0.4^a

0^a

0

E. hallii

1,2-PD

−46.6 ± 6.4^B

1.0 ± 0.8^A

− 1.6 ± 7.8^A

19.3 ± 8.0^B

4.3 ± 0.7^B

n.d.

glycerol

− 13.3 ± 3.0^b

0.7 ± 1.0^b

− 4.6 ± 2.5^b

0 ± 0.8^a

0 ± 0.8^a

1.9 ± 0.7^a

F. prausnitzii

1,2-PD

− 0.7^C

0^A

−1.4 ^A

0^C

1.5^A

n.d.

glycerol

−1.6 ± 0.4^c

0.9 ± 1.6^a

1.3 ± 0.5^a

0.3 ± 0.2^a

0.3 ± 0.6^a

0 ^a

F. plautii

1,2-PD

−48.1 ± 1.2^B

0^A

2.2 ± 1.4^A

11.6 ± 3.9^B

4.1 ± 0.3^B

n.d.

glycerol

−9.1 ± 3.0^c

0^a

5.2 ± 0.7^c

−0.5 ± 0.4^a

4.3 ± 0.5^b

1.2 ± 0.7^a

I. butyriciproduens

1,2-PD

−1.2 ± 2.9^C

0^A

2.6 ± 2.6^A

− 0.1 ± 0.5^C

5.4 ± 0.6^B

n.d.

glycerol

− 0.8 ± 1.4^ac

0^a

2.5 ± 1.7^ac

−0.1 ± 0.1^a

5.3 ± 0.5^b

3.0 ± 0.1^b

L. reuteri

1,2-PD

−9.8 ± 4.2^C

0^A

−1.0 ± 1.1^A

0.9 ± 0.5^C

0^A

n.d.

glycerol

−14.4 ± 8.8^b

0^a

−0.4 ± 1.1^a

0^a

0^a

5.2 ± 0^b

R. hominis

1,2-PD

0^D

0^A

0^A

0^C

0^A

n.g.

glycerol

−5.9 ± 1.1^c

0^a

−8.5 ± 1.3^d

−0.5 ± 0.2^a

7.4 ± 1.6^b

0

R. intestinalis

1,2-PD

−0.4 ± 0.4^C

0^A

− 0.4 ± 0.4^A

−0.3 ± 0.1^C

0.4 ± 0.2^A

n.d.

glycerol

−1.4 ± 0.6^ac

0^a

1.3 ± 1.0^a

0.4 ± 0.4^a

0.4 ± 0.2^a

0^a

R. gnavus

1,2-PD

−18.0 ± 5.1^A

1.1 ± 1.9^A

2.2 ± 1.4^AB

7.9 ± 2.4^A

0.2 ± 0.3^A

n.d.

glycerol

− 1.4 ± 1.2^a

0^a

0.8 ± 1.4^ae

0^a

0^a

0.2 ± 0.3^a

V. dispar

1,2-PD

−1.7 ± 2.4^D

1.4 ± 1.6^A

0 ± 2.3^AB

0.6 ± 0.8^C

0^A

n.d.

glycerol

−4.4 ± 1.4^ac

6.6 ± 1.2^c

−2.4 ± 1.9^be

− 0.5 ± 0.5^a

−0.2 ± 0.4^a

0^a

B. fragilis

1,2-PD

−1.0 ± 1.4^C

0^A

−0.1 ± 1.6^A

0.3 ± 0.5^C

−0.4 ± 0.4^A

n.d.

glycerol

− 1.0 ± 0.9^ac

0^a

0 ± 0.5^a

0.6 ± 0.6^a

0^a

0^a

C. freundii

1,2-PD

−1.4 ± 1.8^D

5.4 ± 0.6^B

7.2 ± 1.2^B

1.5 ± 0.6^D

0^A

n.d.

glycerol

−2.1 ± 3.2^a

6.0 ± 0.5^c

5.3 ± 2.0^c

−0.8 ± 0.8^a

0^a

0^a

K. pneumoniae

1,2-PD

− 42.7 ± 1.1^B

5.3 ± 0.1^B

3.1 ± 1.5^AB

16.7 ± 1.0^B

0^A

n.d.

glycerol

− 48.7 ± 0.8^d

17.5 ± 1.2^d

10.7 ± 0.2^f

0^a

0^a

16.4 ± 3.3^c