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Table 1 Strains used, and presence of glycerol/diol dehydratase (GDH) and β–glucuronidase (B-GUS) encoding genes. The presence of gdh was predicted by metagenome analysis of human feces [24] and was confirmed for the used strains based on genome analysis (https://www.ncbi.nlm.nih.gov/genome/microbes/). The presence of b-gus was predicted by Dabek et al. [26] and McIntosh et al. [27]

From: Gut microbial beta-glucuronidase and glycerol/diol dehydratase activity contribute to dietary heterocyclic amine biotransformation

Strain name

Strain ID

gdh

b-gus

Blautia obeum

DSM 25238

+

Eubacterium eligens

DSM 3376

+

Eubacterium hallii

DSM 3353

+

Faecalibacterium prausnitzii

DSM 17677

+

Flavonifractor plautii

DSM 6740

+

Intestinimonas butyriciproducens

DSM 26588

+

Lactobacillus reuteri

DSM 20016

+

Roseburia hominis

DSM 16839

+

Roseburia intestinalis

DSM 14610

+

Ruminococcus gnavus

ATCC 29149

+

Veillonella dispar

ATCC 17748

+

Bacteroides fragilis

ATCC 25285

+

Citrobacter freundii

CB 36

+

Klebsiella pneumoniae

CB 35

+

  1. +/− gene encoding the indicated enzyme is present/absent in the representative genome. All strains are commercially available except C. freundii and K. pneumoniae, which were obtained in-house collection of Laboratory of Food Biotechnology, ETH Zurich