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Fig. 5 | BMC Microbiology

Fig. 5

From: Gut microbial beta-glucuronidase and glycerol/diol dehydratase activity contribute to dietary heterocyclic amine biotransformation

Fig. 5

Transformation of PhIP-G to PhIP and PhIP-M1 by co-cultures of B-GUS- and GDH-positive strains during growth in YCFA. Transformation of PhIP-G to PhIP and PhIP-M1 by co-cultures of a F. prausnitzii and F. plautii, b F. prausnitzii and L. reuteri, c F. prausnitzii and B. obeum during growth in YCFA with glycerol and glucose (both 50 mM) and PhIP-G (200 nM) at 37 °C for 24 h. PhIP-G, PhIP, and PhIP-M1 were analysed using nanoLC-ESI-MS2. Asterisk indicates significant differences on the relative amount of each compound at 24 h and immediately (0 h) after incubation using two-way ANOVA Sidak’s multiple comparisons test: ** p < 0.01, *** p < 0.001. The B-GUS activity (blue circle) of the bacterial cell extracts was tested with PNP-G

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