Fig. 4
Transformation of PhIP-N2-β-D-glucuronide (PhIP-G) by β-glucuronidase (B-GUS) positive strains during growth in YCFA. Representative chromatographs of PhIP-G and its metabolites PhIP or PhIP-M1 during growth of (A) F. prausnitzii. (B) Relative amount of PhIP-G, PhIP and PhIP-M1 during growth of: Fpr. F. prausnitzii (n = 6), Rh. R. hominis (n = 4), and Ri. R. intestinalis (n = 2) in YCFA with glycerol and glucose (50 mM) and PhIP-G (200 nM) at 37 °C for 24 h. PhIP-G, PhIP, and PhIP-M1 were analysed using nanoLC-ESI-MS2. The B-GUS activity (blue circle) of the bacterial cell extracts was tested with PNP-G. Main transitions are indicated