Fig. 4

Whole genome synteny analysis of pbunaviruses genomes. Schematic genome alignment diagram obtained using the Mauve software package and plotted using genoPlotR. The analysis was performed using 20 complete genomes available in Genbank, as described in Table 3, and with the phage BrSP1 genome. To facilitate synteny analysis, the genomes were oriented to start at the terminase gene (highlighted in black) to overcome the uncertainties in the genome start of several isolates. Gray shading indicates homologous regions among genomes, colored bars represent nucleotide syntenic blocks, and the white spaces between blocks indicate missing regions. All viruses were collinear. The PB1-ORF070 is annotated and highlighted in black