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Fig. 3 | BMC Microbiology

Fig. 3

From: Rifampin resistance and its fitness cost in Riemerella anatipestifer

Fig. 3

In vitro evaluation of the susceptibility of the wild-type ATCC 11845, mutants R494K and H491D to oxidative damage and nitrification stress and their ability to colonize ducklings. a and b Colonization capacity of wild-type ATCC 11845 and the mutants R494K and H491D in the duck brain and trachea. Values are the means of four independent experiments, and the results are shown as the relative number of CFU per gram of organs. c Effect of R494K and H491D substitutions on the sensitivity to oxidative damage. d Effect of R494K and H491D substitutions on the sensitivity to nitrificative stress. When the SNP concentration reached 0.06 mmol/L, none of the three strains could grow. Data are shown as the mean and SDs from four independent experiments, each with triplicate samples. The statistical significance of all the above tests was evaluated by Student’s t-test. The asterisk represents statistical significance (*, P < 0.05; **, P < 0.01)

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