Table 1 Strains, plasmids and primers used in this study
Strains, plasmids and primers | Feature | Source |
|---|---|---|
Strains | ||
Xac29–1 | G−, wt | Ye et al. (2013) |
Xac29–1Δhfq | G−, Δhfq | This study |
Xac 9-1Δhfq-C | G−, hfq+ | This study |
JM109 | G− | Takara |
Plasmids | ||
pEASY-T1 | Kan+/Amp+ | TransGen |
pK18mobSacB | Kan+, SacB | Zou et al. (2011) |
pK18mobSacB-Δhfq | Kan+, SacB, hfq+ | This study |
pBBR1MCS-5 | Gm+ | Zou et al. (2011) |
Primers | ||
hfq-F | 5′-GCTTCAGGCGTGTAACATCC-3’ | This study |
hfq-R | 5′-GCGAACTCCTCCAACACATC-3’ | This study |
hfq-up-F | 5′-TGGGATCCCGCGTGTTGAAGGTGGTATT-3’ | This study |
hfq-up-R | 5′-TGGGTACCCGAAAAATCCTCTTCATTATTGT-3’ | This study |
hfq-down-F | 5′-TGGGTACCCGGAGTAGTGCGTGTTTGATC-3’ | This study |
hfq-down-R | 5′-GCTCTAGAAAGCCTCTGCACCGGTCAACA-3’ | This study |
hfq-F1 | ATGGCTAAGGGGCAATCTTTAC | This study |
hfq-R1 | ACCGATCAAACACGCACTACT | This study |