Skip to main content

Advertisement

Table 1 Strains, plasmids and primers used in this study

From: Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri

Strains, plasmids and primers Feature Source
Strains
Xac29–1 G, wt Ye et al. (2013)
Xac29–1Δhfq G, Δhfq This study
Xac 9-1Δhfq-C G, hfq+ This study
 JM109 G Takara
Plasmids
 pEASY-T1 Kan+/Amp+ TransGen
 pK18mobSacB Kan+, SacB Zou et al. (2011)
 pK18mobSacBhfq Kan+, SacB, hfq+ This study
 pBBR1MCS-5 Gm+ Zou et al. (2011)
Primers
hfq-F 5′-GCTTCAGGCGTGTAACATCC-3’ This study
hfq-R 5′-GCGAACTCCTCCAACACATC-3’ This study
hfq-up-F 5′-TGGGATCCCGCGTGTTGAAGGTGGTATT-3’ This study
hfq-up-R 5′-TGGGTACCCGAAAAATCCTCTTCATTATTGT-3’ This study
hfq-down-F 5′-TGGGTACCCGGAGTAGTGCGTGTTTGATC-3’ This study
hfq-down-R 5′-GCTCTAGAAAGCCTCTGCACCGGTCAACA-3’ This study
hfq-F1 ATGGCTAAGGGGCAATCTTTAC This study
hfq-R1 ACCGATCAAACACGCACTACT This study