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Table 1 Strains, plasmids and primers used in this study
| Strains, plasmids and primers | Feature | Source |
|---|---|---|
| Strains | ||
| Xac29–1 | G−, wt | Ye et al. (2013) |
| Xac29–1Δhfq | G−, Δhfq | This study |
| Xac 9-1Δhfq-C | G−, hfq+ | This study |
| JM109 | G− | Takara |
| Plasmids | ||
| pEASY-T1 | Kan+/Amp+ | TransGen |
| pK18mobSacB | Kan+, SacB | Zou et al. (2011) |
| pK18mobSacB-Δhfq | Kan+, SacB, hfq+ | This study |
| pBBR1MCS-5 | Gm+ | Zou et al. (2011) |
| Primers | ||
| hfq-F | 5′-GCTTCAGGCGTGTAACATCC-3’ | This study |
| hfq-R | 5′-GCGAACTCCTCCAACACATC-3’ | This study |
| hfq-up-F | 5′-TGGGATCCCGCGTGTTGAAGGTGGTATT-3’ | This study |
| hfq-up-R | 5′-TGGGTACCCGAAAAATCCTCTTCATTATTGT-3’ | This study |
| hfq-down-F | 5′-TGGGTACCCGGAGTAGTGCGTGTTTGATC-3’ | This study |
| hfq-down-R | 5′-GCTCTAGAAAGCCTCTGCACCGGTCAACA-3’ | This study |
| hfq-F1 | ATGGCTAAGGGGCAATCTTTAC | This study |
| hfq-R1 | ACCGATCAAACACGCACTACT | This study |
