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Table 1 Strains, plasmids and primers used in this study

From: Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri

Strains, plasmids and primers Feature Source
Strains
 Xac29–1 G−, wt Ye et al. (2013)
 Xac29–1Δhfq G−, Δhfq This study
 Xac 9-1Δhfq-C G−, hfq+ This study
 JM109 G− Takara
Plasmids
 pEASY-T1 Kan+/Amp+ TransGen
 pK18mobSacB Kan+, SacB Zou et al. (2011)
 pK18mobSacB-Δhfq Kan+, SacB, hfq+ This study
 pBBR1MCS-5 Gm+ Zou et al. (2011)
Primers
 hfq-F 5′-GCTTCAGGCGTGTAACATCC-3’ This study
 hfq-R 5′-GCGAACTCCTCCAACACATC-3’ This study
 hfq-up-F 5′-TGGGATCCCGCGTGTTGAAGGTGGTATT-3’ This study
 hfq-up-R 5′-TGGGTACCCGAAAAATCCTCTTCATTATTGT-3’ This study
 hfq-down-F 5′-TGGGTACCCGGAGTAGTGCGTGTTTGATC-3’ This study
 hfq-down-R 5′-GCTCTAGAAAGCCTCTGCACCGGTCAACA-3’ This study
 hfq-F1 ATGGCTAAGGGGCAATCTTTAC This study
 hfq-R1 ACCGATCAAACACGCACTACT This study
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BMC Microbiology

ISSN: 1471-2180

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