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Table 2 Cq values obtained for 107 C. albicans cells after RT-qPCR of the ITS2 RNAa

From: Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater

RNA extraction method

Storage medium

 

TSB-Gb

RNAlater

RNeasy Mini Kit

12.02 ± 0.74

11.63 ± 0.18

RiboPure Yeast Kit

10.31 ± 0.41

10.48 ± 0.24

NucliSENS easyMAG

11.91 ± 0.51

11.62 ± 0.19

  1. aResults are means and standard deviations of six independent extractions
  2. bTryptic Soy Broth + 15% glycerol
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BMC Microbiology

ISSN: 1471-2180

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