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Fig. 2 | BMC Microbiology

Fig. 2

From: Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater

Fig. 2

Percentage of cell lysis obtained after different treatments. a Bead beating during 10 min in a horizontal position. b Horizontal bead beating and vortexing (without beads). c Bead beating in vertical and horizontal position. d Horizontal bead beating and lyticase treatment. e Bead beating in horizontal position after different storage conditions

Error bars represent the standard deviations of results from six biological replicates. Statistically significant differences are marked with an asterisk (P < 0.05) (pair samples Wilcoxon test) and with one (P < 0.05) or two (P < 0.005) bracketed asterisks (Friedman test). a: RLT buffer (RNeasy Mini Kit) + 1% β-mercaptoethanol. b: RiboPure Lysis Buffer. c: Lyticase Lysis Buffer. d: 2 U/μl lyticase for 1 h. e: Tryptic Soy Broth + 15% glycerol

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BMC Microbiology

ISSN: 1471-2180

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