Fig. 2

Validation of RNASeq data by RT-qPCR. RNASeq (green) and RT-qPCR (blue) data at 6 h post-infection in macrophages. Tested genes: [i] 3 genes (Fig|869,728.6.peg.5909*, 6274, 1473) with significantly increased, [ii] 4 genes (Fig|869,728.6.peg.4093*, 5186, 1728, 355) with significantly decreased and [iii] 2 genes (Fig|869,728.6.peg.5948*, 731) without significantly increased level of expression during macrophage infection (a known virulence factor* was included in each group). B. pseudomallei homologs of these genes are shown in brackets. Internal control: 23S rRNA. Expression data is detailed in Additional file 4: Table S2. Horizontal lines show 2-fold change in expression cut-off. Asterisks on the graph indicate statistical significance