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Table 3 Oligodeoxynucleotides used in this study

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Primers

Sequence (5′-3′)

Description

P0-F

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGTCGACATGAAAAAAAGAAAGAGGCGAAAC

Upstream for pMA0911-wapA-pro-NK construction

P0-R

CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACACTCAAAAAATCTCCACCTTTAAACC

Downstream for pMA0911-wapA-pro-NK construction

P1

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTTGATAGGTGGTATGTTTTCGCTTGAAC

Upstream of PP43

P2

CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACGTGTACATTCCTCTCTTACCTATAATGG

Downstream of PP43

P3

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTTGCCGAATTCCATGAACGAGACTTAAAACG

Upstream of PBcaprE

P4

CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACTCGGTTCCCTCCTCATTTTTATACCAACTTG

Downstream of PBcaprE

P5

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGATCGTCACAATGCGCCATCAAACCG

Upstream of PluxS

P6

CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACGGATCCCACTTTATGGACGCCGCAGTGTCTG

Downstream of PluxS

P7

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTCTATCGAGACACGTTTGGCTGG

Upstream of PgsiB

P8

CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACTTCCTCCTTTAATTGGTGTTGGTTGTTGTATTC

Downstream of PgsiB

P9

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGATCATTTAATTGAAGCGCGCGAAGC

Upstream of PyxiE

P10

CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACGCTCTTCCCGCCTTTCGGACTGTGGGTGG

Downstream of PyxiE

P11

GGGACAGGTAGTATTTTTTGAGAAGATCGTGTACATTCCTCTCTTACCTATAATGG

Downstream for PP43-PHpaII

P12

GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGATCTTCTCAAAAAATACTACCTGTCCC

Upstream of PHpaII

P13

GGGACAGGTAGTATTTTTTGAGAAGATCTAAATCGCTCCTTTTTAGGTGGCACAAATGTG

Downstream or PHpaII-PHpaII-PP43

  1. Note: Homology arms of targeting vectors for gene insertions were underlined