Table 3 Oligodeoxynucleotides used in this study
Primers | Sequence (5′-3′) | Description |
|---|---|---|
P0-F | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGTCGACATGAAAAAAAGAAAGAGGCGAAAC | Upstream for pMA0911-wapA-pro-NK construction |
P0-R | CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACACTCAAAAAATCTCCACCTTTAAACC | Downstream for pMA0911-wapA-pro-NK construction |
P1 | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTTGATAGGTGGTATGTTTTCGCTTGAAC | Upstream of PP43 |
P2 | CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACGTGTACATTCCTCTCTTACCTATAATGG | Downstream of PP43 |
P3 | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTTGCCGAATTCCATGAACGAGACTTAAAACG | Upstream of PBcaprE |
P4 | CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACTCGGTTCCCTCCTCATTTTTATACCAACTTG | Downstream of PBcaprE |
P5 | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGATCGTCACAATGCGCCATCAAACCG | Upstream of PluxS |
P6 | CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACGGATCCCACTTTATGGACGCCGCAGTGTCTG | Downstream of PluxS |
P7 | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTCTATCGAGACACGTTTGGCTGG | Upstream of PgsiB |
P8 | CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACTTCCTCCTTTAATTGGTGTTGGTTGTTGTATTC | Downstream of PgsiB |
P9 | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGATCATTTAATTGAAGCGCGCGAAGC | Upstream of PyxiE |
P10 | CCTTTTAAAGTTTCGCCTCTTTCTTTTTTTCATGTCGACGCTCTTCCCGCCTTTCGGACTGTGGGTGG | Downstream of PyxiE |
P11 | GGGACAGGTAGTATTTTTTGAGAAGATCGTGTACATTCCTCTCTTACCTATAATGG | Downstream for PP43-PHpaII |
P12 | GGCAAGGGTTTAAAGGTGGAGATTTTTTGAGTGATCTTCTCAAAAAATACTACCTGTCCC | Upstream of PHpaII |
P13 | GGGACAGGTAGTATTTTTTGAGAAGATCTAAATCGCTCCTTTTTAGGTGGCACAAATGTG | Downstream or PHpaII-PHpaII-PP43 |