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Table 2 Nattokinase yield under the control of tandem repeats containing whole sequence or core region of PHpaII and PP43

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Single

Whole promoter region

in tandem

Core promoter region

in tandem

Promoter

Activity (FU/mL)

Promoter

Activity (FU/mL)

Promoter

Activity (FU/mL)

PHpaII

110.8 ± 5.2

2PHpaII

199.4 ± 7.1

2CPHpaII

200.8 ± 4.6

3PHpaII

213.3 ± 4.1

3CPHpaII

138.3 ± 3.8

4PHpaII

200.0 ± 2.6

  

PP43

140.5 ± 2.5

2PP43

157.2 ± 4.0

2CPP43

166.7 ± 5.3

3PP43

219.2 ± 7.7

3CPP43

181.7 ± 6.3

4PP43

222.9 ± 4.8

4CPP43

231.7 ± 8.0

  

5CPP43

254.2 ± 5.1