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Table 1 Strains and plasmids used in this study

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Strains or plasmids Description Source Highest yield of NK (U/mL)
Strains
Escherichia coli JM109 RecA1 pupE44 endA1 hsdR17 gyrA96 relA1 thi∆(lac-proAB) F′[traD36 proAB+lacIq lacZ∆M15] Lab stock
Bacillus subtilis WB800 nprE aprE epr bpr mpr::ble nprB::bsrvpr wprA::hyg Lab stock
Plasmids
 pMA0911-pro-NK shuttle vector for E. coli/B. subtilis, PHpaII, SPwapA, pro-NK, Apr, Kmr, Lab stock 110.8 ± 5.2
 pSG-pro-NK pMA0911-pro-NK without promoter PHpaII This study
 pSG-PBcaprE pSG-pro-NK with promoter PBcaprE This study 103.5 ± 4.2
 pSG-PluxS pSG-pro-NK with promoter PluxS This study 99.2 ± 3.8
 pSG-PgsiB pSG-pro-NK with promoter PgsiB This study 44.6 ± 2.9
 pSG-PyxiE pSG-pro-NK with promoter PyxiE This study 20.2 ± 2.0
 pSG-PP43 pSG-pro-NK with promoter PP43 This study 140.5 ± 2.5
 pSG-2PgsiB pSG-pro-NK with promoter PgsiB-PgsiB This study 48.0 ± 2.2
 pSG-2PBcaprE pSG-pro-NK with promoter PBcaprE-PBcaprE This study 120.3 ± 2.4
 pSG-2PHpaII pSG-pro-NK with promoter PHpaII-PHpaII This study 199.4 ± 7.1
 pSG-2PP43 pSG-pro-NK with promoter PP43-PP43 This study 157.2 ± 4.0
 pSG-PP43-PHpaII pSG-pro-NK with promoter PP43-PHpaII This study 231.7 ± 6.0
 pSG-PHpaII-PP43 pSG-pro-NK with promoter PHpaII-PP43 This study 210.6 ± 5.2
 pSG-PBcaprE-PHpaII pSG-pro-NK with promoter PBcaprE-PHpaII This study 175.5 ± 5.0
 pSG-PHpaII-PBcaprE pSG-pro-NK with promoter PHpaII-PBcaprE This study 0
 pSG-PyxiE-PHpaII pSG-pro-NK with promoter PyxiE-PHpaII This study 0
 pSG-PHpaII-PyxiE pSG-pro-NK with promoter PHpaII-PyxiE This study 166.7 ± 2.5
 pSG-PgsiB-PHpaII pSG-pro-NK with promoter PgsiB-PHpaII This study 164.9 ± 3.0
 pSG-PHpaII-PgsiB pSG-pro-NK with promoter PHpaII-PgsiB This study 0
 pSG-PluxS-PHpaII pSG-pro-NK with promoter PluxS-PHpaII This study 77.5 ± 4.0
 pSG-PHpaII-PluxS pSG-pro-NK with promoter PHpaII-PluxS This study 0
 pSG-3PHpaII pSG-pro-NK with promoter PHpaII-PHpaII-PHpaII This study 213.3 ± 4.1
 pSG-3PP43 pSG-pro-NK with promoter PP43-PP43-PP43 This study 219.2 ± 7.7
 pSG-2PHpaII-PP43 pSG-pro-NK with promoter PHpaII-PHpaII-PP43 This study 264.2 ± 7.0
 pSG-PP43-2PHpaIII pSG-pro-NK with promoter PP43-PHpaII-PHpaII This study 47.5 ± 3.1
 pSG-PHpaII-2PP43 pSG-pro-NK with promoter PHpaII-PP43-PP43 This study 199.4 ± 7.1
 pSG-2PP43-PHpaII pSG-pro-NK with promoter PP43-PP43-PHpaII This study 149.4 ± 5.0
 pSG-PHpaII-PP43-PHpaII pSG-pro-NK with promoter PHpaII-PP43-PHpaII This study 206.3 ± 7.0
 pSG-PP43-PHpaII-PP43 pSG-pro-NK with promoter PP43-PHpaII-PP43 This study 182.3 ± 5.6
 pSG-4PHpaII pSG-pro-NK with promoter PHpaII-PHpaII-PHpaII-PHpaII This study 200.0 ± 2.6
 pSG-4PP43 pSG-pro-NK with promoter PP43-PP43-PP43-PP43 This study 222.9 ± 4.8
 pSG- 2CPBcaprE pSG-pro-NK with promoter CPBcaprE-PBcaprE This study 120.3 ± 2.4
 pSG-2CPHpaII pSG-pro-NK with promoter CPHpaII-PHpaII This study 200.8 ± 4.6
 pSG-3CPHpaII pSG-pro-NK with promoter CPHpaII-CPHpaII-PHpaII This study 138.3 ± 3.8
 pSG-2CPP43 pSG-pro-NK with promoter CPP43-PP43 This study 166.7 ± 5.3
 pSG-3CPP43 pSG-pro-NK with promoter CPP43-CPP43-PP43 This study 181.7 ± 6.3
 pSG-4CPP43 pSG-pro-NK with promoter CPP43-CPP43-CPP43-PP43 This study 231.7 ± 8.0
 pSG-5CPP43 pSG-pro-NK with promoter CPP43-CPP43-CPP43-CPP43-PP43 This study 254.2 ± 5.1
  1. Note: The corresponding highest yield of NK for each construct was detected using the 36-h supernatant