Strains or plasmids | Description | Source | Highest yield of NK (U/mL) |
---|---|---|---|
Strains | |||
 Escherichia coli JM109 | RecA1 pupE44 endA1 hsdR17 gyrA96 relA1 thi∆(lac-proAB) F′[traD36 proAB+lacIq lacZ∆M15] | Lab stock | – |
 Bacillus subtilis WB800 | nprE aprE epr bpr mpr::ble nprB::bsr ∆vpr wprA::hyg | Lab stock | – |
Plasmids | |||
 pMA0911-pro-NK | shuttle vector for E. coli/B. subtilis, PHpaII, SPwapA, pro-NK, Apr, Kmr, | Lab stock | 110.8 ± 5.2 |
 pSG-pro-NK | pMA0911-pro-NK without promoter PHpaII | This study | – |
 pSG-PBcaprE | pSG-pro-NK with promoter PBcaprE | This study | 103.5 ± 4.2 |
 pSG-PluxS | pSG-pro-NK with promoter PluxS | This study | 99.2 ± 3.8 |
 pSG-PgsiB | pSG-pro-NK with promoter PgsiB | This study | 44.6 ± 2.9 |
 pSG-PyxiE | pSG-pro-NK with promoter PyxiE | This study | 20.2 ± 2.0 |
 pSG-PP43 | pSG-pro-NK with promoter PP43 | This study | 140.5 ± 2.5 |
 pSG-2PgsiB | pSG-pro-NK with promoter PgsiB-PgsiB | This study | 48.0 ± 2.2 |
 pSG-2PBcaprE | pSG-pro-NK with promoter PBcaprE-PBcaprE | This study | 120.3 ± 2.4 |
 pSG-2PHpaII | pSG-pro-NK with promoter PHpaII-PHpaII | This study | 199.4 ± 7.1 |
 pSG-2PP43 | pSG-pro-NK with promoter PP43-PP43 | This study | 157.2 ± 4.0 |
 pSG-PP43-PHpaII | pSG-pro-NK with promoter PP43-PHpaII | This study | 231.7 ± 6.0 |
 pSG-PHpaII-PP43 | pSG-pro-NK with promoter PHpaII-PP43 | This study | 210.6 ± 5.2 |
 pSG-PBcaprE-PHpaII | pSG-pro-NK with promoter PBcaprE-PHpaII | This study | 175.5 ± 5.0 |
 pSG-PHpaII-PBcaprE | pSG-pro-NK with promoter PHpaII-PBcaprE | This study | 0 |
 pSG-PyxiE-PHpaII | pSG-pro-NK with promoter PyxiE-PHpaII | This study | 0 |
 pSG-PHpaII-PyxiE | pSG-pro-NK with promoter PHpaII-PyxiE | This study | 166.7 ± 2.5 |
 pSG-PgsiB-PHpaII | pSG-pro-NK with promoter PgsiB-PHpaII | This study | 164.9 ± 3.0 |
 pSG-PHpaII-PgsiB | pSG-pro-NK with promoter PHpaII-PgsiB | This study | 0 |
 pSG-PluxS-PHpaII | pSG-pro-NK with promoter PluxS-PHpaII | This study | 77.5 ± 4.0 |
 pSG-PHpaII-PluxS | pSG-pro-NK with promoter PHpaII-PluxS | This study | 0 |
 pSG-3PHpaII | pSG-pro-NK with promoter PHpaII-PHpaII-PHpaII | This study | 213.3 ± 4.1 |
 pSG-3PP43 | pSG-pro-NK with promoter PP43-PP43-PP43 | This study | 219.2 ± 7.7 |
 pSG-2PHpaII-PP43 | pSG-pro-NK with promoter PHpaII-PHpaII-PP43 | This study | 264.2 ± 7.0 |
 pSG-PP43-2PHpaIII | pSG-pro-NK with promoter PP43-PHpaII-PHpaII | This study | 47.5 ± 3.1 |
 pSG-PHpaII-2PP43 | pSG-pro-NK with promoter PHpaII-PP43-PP43 | This study | 199.4 ± 7.1 |
 pSG-2PP43-PHpaII | pSG-pro-NK with promoter PP43-PP43-PHpaII | This study | 149.4 ± 5.0 |
 pSG-PHpaII-PP43-PHpaII | pSG-pro-NK with promoter PHpaII-PP43-PHpaII | This study | 206.3 ± 7.0 |
 pSG-PP43-PHpaII-PP43 | pSG-pro-NK with promoter PP43-PHpaII-PP43 | This study | 182.3 ± 5.6 |
 pSG-4PHpaII | pSG-pro-NK with promoter PHpaII-PHpaII-PHpaII-PHpaII | This study | 200.0 ± 2.6 |
 pSG-4PP43 | pSG-pro-NK with promoter PP43-PP43-PP43-PP43 | This study | 222.9 ± 4.8 |
 pSG- 2CPBcaprE | pSG-pro-NK with promoter CPBcaprE-PBcaprE | This study | 120.3 ± 2.4 |
 pSG-2CPHpaII | pSG-pro-NK with promoter CPHpaII-PHpaII | This study | 200.8 ± 4.6 |
 pSG-3CPHpaII | pSG-pro-NK with promoter CPHpaII-CPHpaII-PHpaII | This study | 138.3 ± 3.8 |
 pSG-2CPP43 | pSG-pro-NK with promoter CPP43-PP43 | This study | 166.7 ± 5.3 |
 pSG-3CPP43 | pSG-pro-NK with promoter CPP43-CPP43-PP43 | This study | 181.7 ± 6.3 |
 pSG-4CPP43 | pSG-pro-NK with promoter CPP43-CPP43-CPP43-PP43 | This study | 231.7 ± 8.0 |
 pSG-5CPP43 | pSG-pro-NK with promoter CPP43-CPP43-CPP43-CPP43-PP43 | This study | 254.2 ± 5.1 |