Skip to main content

Table 1 Strains and plasmids used in this study

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Strains or plasmids

Description

Source

Highest yield of NK (U/mL)

Strains

 Escherichia coli JM109

RecA1 pupE44 endA1 hsdR17 gyrA96 relA1 thi∆(lac-proAB) F′[traD36 proAB+lacIq lacZ∆M15]

Lab stock

–

 Bacillus subtilis WB800

nprE aprE epr bpr mpr::ble nprB::bsr ∆vpr wprA::hyg

Lab stock

–

Plasmids

 pMA0911-pro-NK

shuttle vector for E. coli/B. subtilis, PHpaII, SPwapA, pro-NK, Apr, Kmr,

Lab stock

110.8 ± 5.2

 pSG-pro-NK

pMA0911-pro-NK without promoter PHpaII

This study

–

 pSG-PBcaprE

pSG-pro-NK with promoter PBcaprE

This study

103.5 ± 4.2

 pSG-PluxS

pSG-pro-NK with promoter PluxS

This study

99.2 ± 3.8

 pSG-PgsiB

pSG-pro-NK with promoter PgsiB

This study

44.6 ± 2.9

 pSG-PyxiE

pSG-pro-NK with promoter PyxiE

This study

20.2 ± 2.0

 pSG-PP43

pSG-pro-NK with promoter PP43

This study

140.5 ± 2.5

 pSG-2PgsiB

pSG-pro-NK with promoter PgsiB-PgsiB

This study

48.0 ± 2.2

 pSG-2PBcaprE

pSG-pro-NK with promoter PBcaprE-PBcaprE

This study

120.3 ± 2.4

 pSG-2PHpaII

pSG-pro-NK with promoter PHpaII-PHpaII

This study

199.4 ± 7.1

 pSG-2PP43

pSG-pro-NK with promoter PP43-PP43

This study

157.2 ± 4.0

 pSG-PP43-PHpaII

pSG-pro-NK with promoter PP43-PHpaII

This study

231.7 ± 6.0

 pSG-PHpaII-PP43

pSG-pro-NK with promoter PHpaII-PP43

This study

210.6 ± 5.2

 pSG-PBcaprE-PHpaII

pSG-pro-NK with promoter PBcaprE-PHpaII

This study

175.5 ± 5.0

 pSG-PHpaII-PBcaprE

pSG-pro-NK with promoter PHpaII-PBcaprE

This study

0

 pSG-PyxiE-PHpaII

pSG-pro-NK with promoter PyxiE-PHpaII

This study

0

 pSG-PHpaII-PyxiE

pSG-pro-NK with promoter PHpaII-PyxiE

This study

166.7 ± 2.5

 pSG-PgsiB-PHpaII

pSG-pro-NK with promoter PgsiB-PHpaII

This study

164.9 ± 3.0

 pSG-PHpaII-PgsiB

pSG-pro-NK with promoter PHpaII-PgsiB

This study

0

 pSG-PluxS-PHpaII

pSG-pro-NK with promoter PluxS-PHpaII

This study

77.5 ± 4.0

 pSG-PHpaII-PluxS

pSG-pro-NK with promoter PHpaII-PluxS

This study

0

 pSG-3PHpaII

pSG-pro-NK with promoter PHpaII-PHpaII-PHpaII

This study

213.3 ± 4.1

 pSG-3PP43

pSG-pro-NK with promoter PP43-PP43-PP43

This study

219.2 ± 7.7

 pSG-2PHpaII-PP43

pSG-pro-NK with promoter PHpaII-PHpaII-PP43

This study

264.2 ± 7.0

 pSG-PP43-2PHpaIII

pSG-pro-NK with promoter PP43-PHpaII-PHpaII

This study

47.5 ± 3.1

 pSG-PHpaII-2PP43

pSG-pro-NK with promoter PHpaII-PP43-PP43

This study

199.4 ± 7.1

 pSG-2PP43-PHpaII

pSG-pro-NK with promoter PP43-PP43-PHpaII

This study

149.4 ± 5.0

 pSG-PHpaII-PP43-PHpaII

pSG-pro-NK with promoter PHpaII-PP43-PHpaII

This study

206.3 ± 7.0

 pSG-PP43-PHpaII-PP43

pSG-pro-NK with promoter PP43-PHpaII-PP43

This study

182.3 ± 5.6

 pSG-4PHpaII

pSG-pro-NK with promoter PHpaII-PHpaII-PHpaII-PHpaII

This study

200.0 ± 2.6

 pSG-4PP43

pSG-pro-NK with promoter PP43-PP43-PP43-PP43

This study

222.9 ± 4.8

 pSG- 2CPBcaprE

pSG-pro-NK with promoter CPBcaprE-PBcaprE

This study

120.3 ± 2.4

 pSG-2CPHpaII

pSG-pro-NK with promoter CPHpaII-PHpaII

This study

200.8 ± 4.6

 pSG-3CPHpaII

pSG-pro-NK with promoter CPHpaII-CPHpaII-PHpaII

This study

138.3 ± 3.8

 pSG-2CPP43

pSG-pro-NK with promoter CPP43-PP43

This study

166.7 ± 5.3

 pSG-3CPP43

pSG-pro-NK with promoter CPP43-CPP43-PP43

This study

181.7 ± 6.3

 pSG-4CPP43

pSG-pro-NK with promoter CPP43-CPP43-CPP43-PP43

This study

231.7 ± 8.0

 pSG-5CPP43

pSG-pro-NK with promoter CPP43-CPP43-CPP43-CPP43-PP43

This study

254.2 ± 5.1

  1. Note: The corresponding highest yield of NK for each construct was detected using the 36-h supernatant