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Fig. 5 | BMC Microbiology

Fig. 5

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Fig. 5

Effects of the multi core regions of PHpaII and PP43 in tandem on NK production. a The fibrinolytic activities of NK in the supernatant. Recombinant strains harboring promoters of repetitive core regions were cultured in TB medium for 36 h, and then cells and the supernatant culture were separated by centrifugation. The SDS-PAGE analysis of the NK expression mediated by the repetitive core regions of PHpaII (b) and PP43 (c). The arrow indicates the NK bands corresponding to the 36-h supernatant, and 15 μL supernatant was loaded into each lane

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