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Fig. 3 | BMC Microbiology

Fig. 3

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Fig. 3

Overproduction of NK under the control of the dual-promoter systems. a Fibrinolytic activities of NK in the supernatant. b SDS-PAGE analysis. Lane M: standard marker proteins. The position of the NK protein bands is indicated by an arrow. Recombinant strains having different dual-promotes were cultured in the TB medium for 36 h, and then the cells and the supernatant culture were separated by centrifugation

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