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Fig. 1 | BMC Microbiology

Fig. 1

From: High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

Fig. 1

Schematic representation of the expression cassettes. a Map of the pSG(x)-NK vectors. All of the expression cassettes were cloned into the pMA0911-wapA-pro-NK, and the sites of the relevant restriction enzymes were shown. b The schematic diagram of the expression cassettes with tandem promoters. The signal peptide (SP) and the NK gene are represented by gray and black, respectively. The promoters, PX, are represented by arrows. c The expression cassettes with repetitive core regions of promoters. The sequences of core regions (− 35 and − 10) are shown

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