Diversity and community of methanogens in the large intestine of finishing pigs

BMC Microbiology

Table 5 The characteristics of the primer and probe sets used in this study

Target group	Function	Sequence (5′-3′)	Tm (°C)	Amplicon Size (bp)	References
Methanogens	F primer	GGTGGTGTMGGATTCACACARTAYGCWACAG	58	470	Luton et al. (2002) [33]
Methanogens	R primer	TTCATTGCRTAGTTWGGRTAGTT	58	470	Luton et al. (2002) [33]
Methanobacteriales	F primer	GCCATGCACCWCCTCT	62	343	Yu et al. (2005) [39]
Methanobacteriales	R primer	TACCGTCGTCCACTCCTT	62	343	Yu et al. (2005) [39]

ISSN: 1471-2180