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Fig. 7 | BMC Microbiology

Fig. 7

From: Infection and nuclear interaction in mammalian cells by ‘Candidatus Berkiella cookevillensis’, a novel bacterium isolated from amoebae

Fig. 7

Detection of lamina gaps in nuclei of ‘Ca. Berkiella cookevillensis’-treated HeLa cells. Indirect immunofluorescence staining of HeLa cells infected with ‘Ca. B. cookevillensis (CC99) at an MOI of 200 for 24 h. HeLa cells were labeled using an anti-lamin A/C antibody and co-stained with DAPI. a-c Three examples of CC99-rich vacuoles invading the host cell nucleus. The DAPI + lamin merged micrograph demonstrates the position of bacterial DNA to the nuclear lamina. The fluorescence intensity profile for the lamin stain was constructed to quantify the size of the nuclear lamina gap (red arrow). Bars, 2 μm

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