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Table 2 Biochemical test results of conventional culture method after 24 h incubation

From: Isolation and identification of microflora from the midgut and salivary glands of Anopheles species in malaria endemic areas of Ethiopia

Anopheles species

Abdominal condition

Sources of samples

Biochemical tests

Additional

Identified bacteria

LDC

CIT

TSIA

SIM

UREA

H2S

Motility

Indole

Anopheles funestus

Fed Gravid Unfed

Midgut Salivary gland

-ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi+

Pseudomonas spp.††

Gravid

S. gland

-ve

+ve

+ve

+ve

-ve

+ve

+ve

Cat+, Oxi−

Citrobacter spp.††

Anopheles pharoensis

Fed

S. gland

-ve

+ve

-ve

-ve

-ve

+ve

-ve

Cat+, Oxi−

Klebsiella ozane ††

-ve

+ve

-ve

+ve

-ve

+ve

-ve

Cat+, Oxi−

Providencia rettgeri ††

Gravid Unfed Unfed

Midgut

-ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi+

Pseudomonas spp.††

S. gland

+ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi−

Acinetobacter spp.††

Anopheles gambiae s.l

Fed

S. gland

-ve

+ve

+ve

+ve

-ve

+ve

-ve

Cat+, Oxi−

Enterobacter cloacae ††

Fed

Midgut S. gland

-ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi+

Pseudomonas spp.††

Gravid

Midgut S. gland

-ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi+

Pseudomonas spp.††

S. gland

+ve

+ve

+ve

-ve

-ve

+ve

-ve

Cat+, Oxi−

Klebsiella pneumonia ††

Unfed

Midgut

-ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi+

Pseudomonas spp.††

S. gland

+ve

+ve

+ve

-ve

-ve

+ve

-ve

Cat+, Oxi−

Klebsiella pneumonia ††

Anopheles zeimanni

Fed

Midgut

+ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi−

Acinetobacter spp.††

Fed unfed gravid

Midgut S. gland

-ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi+

Pseudomonas spp.††

Gravid Unfed

S. gland Midgut

+ve

+ve

+ve

-ve

-ve

+ve

-ve

Cat+, Oxi−

Klebsiella pneumonia ††

Gravid

S. gland

-ve

+ve

+ve

+ve

-ve

+ve

+ve

Cat+, Oxi−

Citrobacter spp.††

+ve

+ve

-ve

-ve

-ve

-ve

-ve

Cat+, Oxi−

Acinetobacter spp.††

Unfed

Midgut

-ve

+ve

+ve

+ve

-ve

+ve

+ve

Cat+, Oxi−

Citrobacter spp.††

  1. Note: LDC Lysine decarboxylase, CIT Citrate, TSIA Triple Sugar Iron Agar, SIM Sulfur Indole Motility Media, H2S Hydrogen sulfide
  2. †† is gram negative bacteria species/genera