Fig. 4

Excerpt of metabolic network responses of O. polymorpha strains (left hand side) and K. marxianus strains (right hand side) in glycolysis (represented by the the phosphoglucose isomerase reaction), the pentose phosphate pathway (represented by the glucose-6-phosphate dehydrogenase reaction), the tricarboxylic acid cycle (represented by the citrate synthase reaction), and malic enzyme activity to cultivation at higher temperatures. The malic enzyme fluxes producing NADH and NADPH were combined in this representation. Flux values are normalized to the glucose uptake rate. To better illustrate adaptive responses, the fluxes are accentuated with a spline curve fitted to the computed flux data. The reactions are designated by the stoichiometric reaction equation as defined in the model. G6P – glucose-6-phosphate; F6P – fructose-6-phosphate; 6PG – 6-phosphogluconate; ACCOA – acetyl-CoA; OAA – oxaloacetate CIT – citrate; MAL – malate; PYR – pyruvate; mit –mitochondrial localization