Table 9 Histological Characterization of Neighboring Sections of the Materials That Were Used for NGS Assessment. Samples of Cases with Invasive Mycosis Had Been Assessed by HE, Giemsa, PAS, and Grocott Staining [13], Samples of Cases with Invasive Amebiasis by PAS Staining [7]. Especially in the Case of Filamentous Fungi, No Quantification Had Been Attempted, Because Elements of a Multiply-cut Filament Were Indistinguishable from Single Cuts of Multiple Filaments
Sample | Histological diagnosis | Microscopic confirmation of pathogens in neighboring sections of the materials for NGS assessment |
|---|---|---|
1 | Chromoblastomycosis | Microorganisms seen, no quantification performed |
2 | Mucormycosis | Microorganisms seen, no quantification performed |
3 | Histoplasmosis | Microorganisms seen, < 10 pathogens per unit area (160 μm × 210 μm) |
4 | Mucormycosis | Microorganisms seen, no quantification performed |
5 | Histoplasmosis or cryptococcosis | Microorganisms seen, < 10 pathogens per unit area (160 μm × 210 μm) |
6 | Chromoblastomycosis | Microorganisms seen, no quantification performed |
7 | Rhinosporidiosis | Microorganisms seen, no quantification performed |
8 | Mycetoma | Microorganisms seen, no quantification performed |
9 | Rhinosporidiosis | Microorganisms seen, no quantification performed |
10 | Mycetoma | Microorganisms seen, no quantification performed |
11 | Histoplasmosis | Microorganisms seen, < 10 pathogens per unit area (160 μm × 210 μm) |
12 | Histoplasmosis | Microorganisms seen, < 10 pathogens per unit area (160 μm × 210 μm) |
13 | Chromoblastomycosis | Microorganisms seen, no quantification performed |
14 | Histoplasmosis | Microorganisms seen, < 10 pathogens per unit area (160 μm × 210 μm) |
15 | Coccidioidomycosis | Microorganisms seen, no quantification performed |
16 | Coccidioidomycosis | Microorganisms seen, no quantification performed |
17 | Mycetoma | Microorganisms seen, no quantification performed |
18 | Invasive amebiasis | Microorganisms seen, > 5 pathogens per unit area (160 μm × 210 μm) |
19 | Invasive amebiasis | Negative microscopy in neighboring sections |
20 | Invasive amebiasis | Microorganisms seen, > 5 pathogens per unit area (160 μm × 210 μm) |
21 | Invasive amebiasis | Microorganisms seen, < 0.5 pathogens per unit area (160 μm × 210 μm) |
22 | Invasive amebiasis | Negative microscopy in neighboring sections |
23 | Invasive amebiasis | Uncertain microscopic result in neighboring sections |