Fig. 2From: A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity informationAluI restriction profiles of bacterial endophytes (OTUs) from cacao, amplified by 799F and U1492R primers. OTUs were chosen to illustrate the variety of banding patterns and counts observed from individual AluI digestions of the 72 culturable isolates. Amplicons from each OTU (lane) were digested by the 4-bp cutter restriction enzyme and electrophoresed in 5–11% polyacrylamide gradient gel. The ID numbers of isolates (Additional file 1: Table S1) appear at the top. Fragment sizes (in bp) are indicated on the left; ‘L’: 50-bp ladder. Arrows illustrate 5-bp size differences between fragmentsBack to article page