Fig. 1From: A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity informationEffects of chloroplast DNA from Virola officinalis and number of amplification reactions on PCR products from the 16S rRNA gene amplified with primers 799F and U1492R. a Amplification patterns from ‘nested’ and ‘direct’ PCR done on pooled DNA from a 5-OTUs’ mock bacterial community (MBC), with different percentages of amplified/purified V. officinalis cp DNA (see Methods) added to samples prior to the PCR. Agarose and polyacrylamide gels were stained with GelGreen™. ‘L’: size ladder; ‘100’, ‘65’, ‘30’, ‘0’: percentage of cpDNA; ‘C+’: positive control (DNA of a single bacterial isolate); ‘C–’: negative control. b Chloroplast and bacterial 16S rDNA amplification patterns in V. officinalis total leaf-DNA samples from nested PCR; ‘1 to 5’: distinct plant individuals from the same area. c Patterns of nested and direct PCR from the same samples of total DNA extracted from V. officinalis leaves; ‘6 to 9’: distinct plants from same area. d AluI restriction profiles from MBCs amplified with different percentages of cpDNA. ‘L’: size ladder (50 pb); ‘C5’: 5-OTUs’ MBC; ‘C10’: 10-OTUs’ MBC; ‘100’, ‘65’, ‘30’, ‘0’: percentages of cpDNA in the MBCs, prior to PCR, digestion and electrophoresis in 5–11% polyacrylamide gradient gelBack to article page