Fig. 4

Effect of a T7 promoter on Caf1 production at 30 °C and 35 °C. E. coli cells were transformed with either pCOP (full caf1 operon) or pT7-COP (T7- full caf1 operon) and grown at either 30 °C or 35 °C for 16 h, after which the flocculent layer height was measured (a) and analysed by SDS-PAGE (b). Error bars represent standard error of the mean (S.E.M) from three replicate cultures. The position of the band corresponding to Caf1 is highlighted next to the gel