Skip to main content

Advertisement

Table 3 Proteins associated with copper response in P. aeruginosa PAO1 membrane fraction. Protein samples from the copper stressed PAO1 were compared against the control group (+/− 1.5 –fold abundance change, p < 0.01)

From: Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

Protein Fold change (+/−) p-value Peptides Product Description Subcellular Localization (localization [confidence code])* Product Name Confidence
PA2542 42.6 0.003 1 Conserved hypothetical protein Outer Membrane [Class 3] Class 4
PA3351 20.7 0.001 1 FlgM Extracellular [Class 3] Class 1
PA2505 17.9 0.000 13 Tyrosine porin OpdT Outer Membrane [Class 2]; Outer Membrane [Class 3]; Outer Membrane Vesicle [Class 1] Class 1
PA2064 7.2 0.003 9 Copper resistance protein B precursor Outer Membrane [Class 3]; Outer Membrane Vesicle [Class 1] Class 2
PA3920 6.2 0.001 12 Probable metal transporting P-type ATPase Cytoplasmic Membrane [Class 3] Class 3
PA4492 4.7 0.006 1 MagA Unknown [Class 3]; Cytoplasmic Membrane [Class 1] Class 1
PA2520 4.3 0.007 1 Resistance-Nodulation-Cell Division (RND) divalent metal cation efflux transporter CzcA Cytoplasmic Membrane [Class 2]; Cytoplasmic Membrane [Class 3] Class 2
PA5021 2.8 < 0.0001 1 Probable sodium/hydrogen antiporter Cytoplasmic Membrane [Class 3] Class 3
PA4935 2.09 0.009 2 30S ribosomal protein S6 Cytoplasmic [Class 3] Class 2
PA3730 1.67 0.009 1 Hypothetical protein Cytoplasmic Membrane [Class 3] Class 4
PA1766 1.64 0.006 4 Hypothetical protein Cytoplasmic [Class 3] Class 4
PA3459 −1.50 0.005 4 Probable glutamine amidotransferase Cytoplasmic [Class 3] Class 3
PA3731 −1.53 0.005 6 Conserved hypothetical protein Cytoplasmic [Class 3] Class 4
PA5174 −1.56 0.007 4 Probable beta-ketoacyl synthase Cytoplasmic [Class 3] Class 3
PA3011 −1.58 0.009 7 DNA topoisomerase I Cytoplasmic [Class 3] Class 2
PA5204 −1.67 0.002 12 N-acetylglutamate synthase Cytoplasmic [Class 3] Class 1
PA3552 −1.79 0.007 5 ArnB Cytoplasmic [Class 3] Class 2
PA4272 −1.86 0.001 3 50S ribosomal protein L10 Cytoplasmic [Class 3] Class 2
PA3769 −1.95 0.001 6 GMP synthase Cytoplasmic [Class 3] Class 2
PA0390 −2.05 0.010 2 homoserine O-acetyltransferase Cytoplasmic [Class 3] Class 2
PA3042 −2.14 0.001 3 Hypothetical protein Unknown [Class 3] Class 4
PA5312 −2.14 0.008 7 Aldehyde dehydrogenase Cytoplasmic [Class 3] Class 2
PA3040 −2.21 0.002 4 Conserved hypothetical protein Unknown [Class 3]; Outer Membrane Vesicle [Class 1] Class 4
PA2345 −2.35 0.002 6 Conserved hypothetical protein Unknown (This protein may have multiple localization sites) [Class 3]; Unknown (This protein may have multiple localization sites) [Class 3] Class 4
PA3952 −2.35 0.002 2 Hypothetical protein Unknown [Class 3] Class 4
PA3746 −2.70 0.001 2 Signal recognition particle protein Ffh Cytoplasmic Membrane [Class 3] Class 2
PA4786 −3.15 0.002 6 Probable short-chain dehydrogenase Cytoplasmic [Class 2]; Cytoplasmic [Class 3] Class 3
PA3183 −3.50 0.008 4 Glucose-6-phosphate 1-dehydrogenase Cytoplasmic [Class 3] Class 1
  1. *Extracted from PGD. Class 1: Subcellular localization experimentally demonstrated in Pseudomonas aeruginosa. Class 2: Subcellular localization of a similar gene experimentally demonstrated in another organism OR to a paralog experimentally demonstrated in the same organism. BLAST expect value of 10e-10 for query within 80–120% of subject length. Class 3: Subcellular localization computationally predicted by PSORTb [44]. Bold indicates first time evidence of protein translation in PAO1