Table 1 Primer sequences used for the amplification of secondary metabolite biosynthetic genes
Sl No | Primer | Sequence (5′ – 3′) | Annealing temperature used (°C) | Approximate product Size (base pairs) |
|---|---|---|---|---|
1 | BaeR-F | AGACTCCACCAAGGCAAATC | 55 | 990 |
BaeR-R | CAGCGGCTTCATGTCATACT | |||
2 | DfnD-F | CAGGCGGAATAGGAGAAGTATG | 54 | 900 |
DfnD-R | CGGCAGCCGATTGAAATAAC | |||
3 | SrfA-F | GCTGATGATGAGGAGAGCTATG | 55 | 890 |
SrfA-R | GATGGTCGATACGTCCGATAAA | |||
4 | MlnA-F | GGCAGGGTCATACCTCATAATC | 55 | 920 |
MlnA-R | AGCAGACTTTCGGTCTCATTC | |||
5 | DhbE-F | GCTGGAGGAAGAGTGGTATTATC | 54 | 940 |
DhbE-R | CAGTAAATGAAGCGGCGTTATG | |||
6 | BacD-F | CCGGCGTCAAGTCTATCAAA | 54 | 670 |
BacD-R | CATGGCTCCTGCTCCAATAA | |||
7 | ItuA-F | CGGGAAACAACAGGCAAATC | 55 | 980 |
ItuA-R | CGTCACCAGCGGTGTAAATA | |||
8 | FenA-F | CATTCATCCTGGAGACCCTATTC | 55 | 960 |
FenA-R | TAAGACCGCAGGCATGTTATAG |