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Fig. 4 | BMC Microbiology

Fig. 4

From: Development of flow cytometry based adherence assay for Neisseria gonorrhoeae using 5′-carboxyfluorosceinsuccidyl ester

Fig. 4

Real Time PCR analysis of neutralization and adherence inhibition assay using ME-180 cells, and ME-180 cells infected with CFSE-stained N. gonorrhoeae (Ng) with and without incubation with serum antibodies. CFSE-stained Ng was preincubated with heat inactivated negative (1:10 and 1:20 dilutions) and positive hyperimmune serum (1:10 to 1:80 dilutions). Infected ME-180 cells were washed three times with PBS to remove loosely adherent CFSE-stained Ng

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