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Table 4 Effects of variations on MRS broth on inhibitory activity of bacteriocinogenic lactic acid bacteria isolated from calabresa against L. monocytogenes 72, assessed by a quantitative assay

From: Lactobacillus curvatus UFV-NPAC1 and other lactic acid bacteria isolated from calabresa, a fermented meat product, present high bacteriocinogenic activity against Listeria monocytogenes

Effect

MRS variant

Concentration (mg/mL)

Bacteriocinogenic strain

L. curvatus 12

L. curvatus 36

W. viridescens 23

Control

MRS

25,600

25,600

25,600

pH

2

200

0

1600

4

25,600

200

1600

6

25,600

25,600

25,600

8

1600

0

1600

10

12,800

0

0

12

25,600

0

0

Carbo-hydrate

lactose

20

1600

0

25,600

sacarose

20

800

0

25,600

mannitol

20

3200

0

800

fructose

20

25,600

0

25,600

dextrose

20

25,600

25,600

25,600

maltose

20

25,600

0

25,600

raffinose

20

12,800

100

25,600

Organic nitrogen

peptone

25

25,600

0

25,600

meat extract

25

25,600

0

25,600

yeast extract

25

25,600

100

25,600

peptone + meat extract

12.5 + 12.5

25,600

100

12,800

peptone + yeast extract

15 + 7.5

25,600

200

25,600

meat extract + yeast extract

15 + 7.5

25,600

100

25,600

peptone + meat extract + yeast extract

10 + 10 + 5

25,600

25,600

25,600

Chemicals

KH2PO4

0

25,600

100

12,800

 

2

25,600

25,600

25,600

 

5

25,600

100

25,600

 

10

25,600

0

25,600

MgSO4

0

25,600

25,600

100

 

0.1

25,600

25,600

25,600

 

0.5

25,600

25,600

100

MnSO4

0

1600

25,600

0

 

0.05

25,600

25,600

25,600

 

0.2

25,600

0

100

Sodium acetate

0

25,600

0

25,600

 

5

25,600

25,600

25,600

 

10

25,600

100

25,600

Tri-ammonium citrate

0

25,600

0

25,600

 

2

25,600

25,600

25,600

 

5

25,600

100

25,600

Tween 80

0

12,800

0

12,800

 

1

25,600

25,600

25,600

 

2

25,600

100

25,600

 

5

25,600

200

25,600