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Fig. 5 | BMC Microbiology

Fig. 5

From: Genome-wide discovery of structured noncoding RNAs in bacteria

Fig. 5

Reporter gene expression is regulated by the thiS riboswitch candidate. a Sequence and predicted secondary structure of the WT thiS RNA associated with the thiS gene of C. maddingley, which was fused to a β-galactosidase reporter gene (lacZ) and a B. subtilis lysC promoter to drive transcription. The lysC promoter was chosen for this purpose because it is known to strongly promote transcription without regulation [73]. Encircled 88 designates the number of additional nucleotides between the end of the terminator element and the lacZ reporter gene sequence. Red nucleotides are > 97% conserved in the thiS consensus model (Fig. 4a). b Reporter gene expression of WT B. subtilis cells and cells lacking the coding region for the ThiS protein (ΔthiS) grown in minimal (GMM) liquid media. c Agar diffusion assay of the ΔthiS B. subtilis strain with a WT riboswitch reporter construct. The filter disk was spotted with 10 mM thiamin on a minimal (GMM) agar medium plate with 100 μg mL− 1 X-Gal

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