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Fig. 2 | BMC Microbiology

Fig. 2

From: Genome-wide discovery of structured noncoding RNAs in bacteria

Fig. 2

Plots of the IGRs from the HIMB5 genome sorted based on IGR length and GC content. a IGR plot prior to conducting our detailed analyses. Red line represents the boundary between unknown IGRs chosen for further analysis (upper right) and those that were not chosen (lower left). IGRs selected for further analysis are depicted with dark gray triangles whereas those not chosen are depicted with light gray triangles. b, (Top) A portion of the sector of interest from the HIMB5 IGR plot after analysis, updated to remove false IGRs that overlap known ORFs, to annotate IGRs carrying previously known ncRNAs, and to include all novel motifs identified in this study. No changes in the plot occurred outside of the area depicted. (Bottom) List of novel motif candidates. c, Summary of the classification of all 47 unknown IGRs from HIMB5 chosen for further analysis. Classifications are organized into five main groups (gray arcs) as annotated, wherein “unknown functions” encompasses categories 1 through 4 (unnamed, LRC, MRC and HRC), and the remaining groups are derived from category 5 (named), as described in the main text. The number of novel examples classified in each group are provided in the colored boxes. Classifications depicted as partially transparent lack a representative in the sector of interest in this genome. See Additional file 1: Table S1 for additional details regarding novel motifs

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