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Table 3 Summary of genetic contexts of blaCTX-M in ESC-resistant E. coli and K. pneumoniae

From: Epidemiology of paediatric gastrointestinal colonisation by extended spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates in north-west Cambodia

  bla CTX-M-15 bla CTX-M-55 bla CTX-M-14 bla CTX-M-27 bla CTX-M-24
EC (n = 41) KP (n = 12) EC (n = 24) KP (n = 0) EC (n = 15) KP (n = 2) EC (n = 12) KP (n = 2) EC (n = 3) KP (n = 0)
Location (chromosome versus plasmid)
 Chromosomal 5 (12%) 0 4 (17%) 2 (13%) 0 0 0 2 (67%)
 Likely plasmid 2 (5%) 9 (75%) 4 (17%) 5 (33%) 2 (100%) 0 0 1 (33%)
 Not determined 34 (83%) 3 (25%) 16 (67%) 8 (53%) 0 12 (100%) 2 (100%) 0
Evaluable immediate flanking context n = 40a n = 12 n = 23c n = 15 n = 2 n = 12 n = 2 n = 3
ISEcp1 upstream 40 (100%) 12 (100%) 23 (100%) 15 (100%) 2 (100%) 12 (100%) 2 (100%) 3 (100%)
 3′ GACTA target site sequence (TSS) at 48 bp upstream of blaCTX-M 36 (90%) 12 (100%) 23 (100%) 0 0 0 0 0
 3′ TTTCA TSS at 127 bp upstream of blaCTX-M 4 (10%) 0 0 0 0 0 0 0
 3′ GAATA TSS at 42 bp 0 0 0 15 (100%) 2 (100%) 12 (100%) 2 (100%) 3 (100%)
ISEcp1 incomplete 26 (65%) 0 14 (61%) 6 (40%) 0 12 (100%) 2 (100%) 1 (100%)
 due to IS26 element 13b (32%) L IRR - 11 R IRR - 2 0 12d (52%) L IRR - 1 R IRR - 11 0 0 12f (100%) L IRR - 12 2 (100%) L IRR - 2 0
 due to contig break 13 (32%) 0 2 (9%) 3 (20%) 0 0 0 1 (33%)
 due to ISVsa5-like 0 0 0 2 (13%) 0 0 0 0
 due IS1S R IRR 0 0 0 1 (7%) 0 0 0 0
ISEcp1 complete 13 (32%) 12 (100%) 9 (39%) 9e(60%) 2 (100%) 0 0 2 (67%)
 5′ TCATA TSS 9 (22%) 12 (100%) 4 (17%) 0 0 0 0 0
 5′ TAATA TSS 4 (10%) 0 3 (13%) 0 0 0 0 0
 5′ TAACA TSS 0 0 2 (14%) 0 0 0 0 0
 5′ CATTA TSS 0 0 0 4 (44%) 0 0 0 1 (33%)
 5′ AAATA TSS 0 0 0 2 (22%) 0 0 0 0
 5′ TAAAA TSS 0 0 0 1 (11%) 0 0 0 0
 5′ GCCGA TSS 0 0 0 1 (11%) 0 0 0 0
 5′ TATAT TSS 0 0 0 0 0 0 0 1 (33%)
 5′ TAGCA TSS 0 0 0 0 2 (100%) 0 0 0
  1. EC = E. coli, KP = K. pneumoniae
  2. aexcluding one isolate with short contigs harbouring truncated blaCTX-M-15
  3. bISEcp1 truncated at 24, 497, 524, 1067, 1173, 1421, or 1489 bp
  4. cexcluding one isolate with short contig harbouring truncated blaCTX-M-55
  5. dISEcp1 truncated at 267, 309 or 497 bp
  6. eFor one only 1 bp of 5′ TSS evaluable
  7. fISEcp1 truncated at 149, 192, 208 and 388 bp
  8. TSS = target site sequence
  9. L IRR = left inverted repeat region
  10. R IRR = left inverted repeat region