Fig. 3
The chromosomal location of Tet P in isolates of C. difficile. Shown are visual representations of blastn alignments. ORFs are colored according to the gene they represent or the predicted function of their product [refer to key in (a)]. Regions of nucleotide identity between sequences are represented by grey bars; the higher the identity, the darker the grey, as illustrated by the legends. Produced using EasyFig [43]. a C. difficile (Cd) isolates that encode Tet P (P12 and MCD46) in comparison to the equivalent chromosomal location in the Tet P negative isolate 08ACCD0030 (for which this region is 99% identical to that of C. difficile type strain 630) and the Tet P+ P. sordellii (Ps) isolate W2945. The cut off for nucleotide identity was a maximum e value of 0.001 with no minimum identity values over a length of 150 bases. The location of an 11 bp region, the sequence for which is shown in the figure, is indicated by green lines on each genome sequence. The one exception is the repeat marked with ‘*’, for which there is a single base pair mismatch. b Comparison displaying the greater genomic context for chromosomally encoded Tet P in C. difficile isolates. Shown are the C. difficile (Cd) isolates in comparison to their relative genomic location in the Tet P− isolate 08ACCD0030 and the Tet P+ P. sordellii (Ps) isolate W2945. The cut off for nucleotide identity was a maximum e value of 0.001 with no minimum identity values over a length of 500 bases