Table 1 Primers used for reverse transcription quantitative polymerase chain reaction (RT-qPCR)

PGN_0557 (hmuR)

TonB-dependent receptor HmuR

Forward

Backward

5′-3′: TAGTCGCGACGGACAGAAAT

5′-3′: CTGGTGAAGATCCCACGTTT

PGN_1058 (ftn)

Ferritin

Forward

Backward

5′-3′: GAAATGATCGAGGCTGTCGT

5′-3′: GTCCTGTGATGCCATATCTCC

PGN_0780 (prtQ)

PrtQ, protease

Forward

Backward

5′-3′: CAGCTGTAAACCGCAACAAG

5′-3′: GGCTTGGCTCCCGTATTATC

PG_0437

Polysaccharide biosynthesis/export protein

Forward

Backward

5′-3′: AGAGGGCCTTACTCGTACCG

5′-3′:CCACTGGAAATAATCCTCTTCTGT

PGN_0183 (fimC)

Minor component FimC

Forward

Backward

5′-3′:CCTTTTCAAGAAAGAACTTGAGGA

5′-3′: GTCGGACTATCGGCTCGTT

PG_2131

OmpA_c-like

Forward

Backward

5′-3′: ACACACCCCTCTCGTCTGAG

5′-3′: TCCCTTCCGGATAGCTCTG

PGN_0181

Fimbrillin-A associated anchor proteins Mfa1 and Mfa2

Forward

Backward

5′-3′: CCACTACGGTGTCTTTCGTG

5′-3′: TTAGACGCTTTGCACATTGG

PG_1712

Alpha-1,2-mannosidase family protein

Forward

Backward

5′-3′: GCTACGAAAGCCGTCCATC

5′-3′: GTACCACTCCCAACCTTTGC