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Table 1 Primers used for reverse transcription quantitative polymerase chain reaction (RT-qPCR)

From: Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells

Locus name Putative identification Primers for RT-qPCR
PGN_0557 (hmuR) TonB-dependent receptor HmuR Forward
Backward
5′-3′: TAGTCGCGACGGACAGAAAT
5′-3′: CTGGTGAAGATCCCACGTTT
PGN_1058 (ftn) Ferritin Forward
Backward
5′-3′: GAAATGATCGAGGCTGTCGT
5′-3′: GTCCTGTGATGCCATATCTCC
PGN_0780 (prtQ) PrtQ, protease Forward
Backward
5′-3′: CAGCTGTAAACCGCAACAAG
5′-3′: GGCTTGGCTCCCGTATTATC
PG_0437 Polysaccharide biosynthesis/export protein Forward
Backward
5′-3′: AGAGGGCCTTACTCGTACCG
5′-3′:CCACTGGAAATAATCCTCTTCTGT
PGN_0183 (fimC) Minor component FimC Forward
Backward
5′-3′:CCTTTTCAAGAAAGAACTTGAGGA
5′-3′: GTCGGACTATCGGCTCGTT
PG_2131 OmpA_c-like Forward
Backward
5′-3′: ACACACCCCTCTCGTCTGAG
5′-3′: TCCCTTCCGGATAGCTCTG
PGN_0181 Fimbrillin-A associated anchor proteins Mfa1 and Mfa2 Forward
Backward
5′-3′: CCACTACGGTGTCTTTCGTG
5′-3′: TTAGACGCTTTGCACATTGG
PG_1712 Alpha-1,2-mannosidase family protein Forward
Backward
5′-3′: GCTACGAAAGCCGTCCATC
5′-3′: GTACCACTCCCAACCTTTGC