Fig. 2
IS256 insertion in the A07 fragment of the SAPIG2195 coding region. a ST398-specific PCR using primers A07f/A07r [30] of different t011 MRSA isolates from farm ID 05CS. Lanes 1 and 2, A07 (197 bp) and A07::IS256 (1535 bp), respectively. Lanes M1 and M2, 1 kb and 100 bp molecular size markers (Promega) respectively. b Schematic of the IS256 element inserted in the A07 fragment of SAPIG2195 [31]. The transposase gene (tnp) is flanked by non-coding regions (NCR-L and NCR–R) that harbor imperfect inverted repeats (IR-L and IR-R). The two octanucleotide direct repeats (DR-L and DR-R) flank the IS256