Fig. 7
Inoculation of Mi in animals expands the frequency of effector and central memory CD8 T cells. Lymphocytes isolated from the lungs of mice injected with Mi and exposed to Mtb were cultured in vitro with PPD. The control group of mice was inoculated with BCG. The cells were then monitored for the expression of activation markers (a) CD62LhiCD44hi; (b) CD62LloCD44hi; (c) CD127hi (d) KLRG1hi on CD8 T cells by flow cytometry. Increase in the percentage of CD62LhiCD44hi, CD62LloCD44hi, CD127hi and KLRG1hi is depicted as flow cytometry histograms (left panel) and bar diagram (right panel). The data are representative of pooled lymphocytes of 4–5 mice/group obtained from triplicate wells. *p < 0.05, **p < 0.005, ***p < 0.0005