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Table 2 Detection of E. hirae in hearts of chickens representing different disease outbreaks

From: The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

No. of affected heart LAMP – cells/g CFU counting LAMP – GE/g
H3 1.74 × 108 4.0 × 106 1.84 × 107
H7 1.13 × 108 5.0 × 106 1.2 × 107
H2 8.81 × 107 3.53 × 107 9.35 × 106
H4 6.91 × 107 2.0 × 106 7.34 × 106
H5 1.86 × 107 8.0 × 106 1.97 × 106
H1 4.84 × 106 3.3 × 106 5.14 × 105
H9 6.22 × 104 7.67 × 102 6.6 × 103
H6 9.36 × 103 2.33 × 105 9.94 × 102
H8 1.99 × 103 1.0 × 104 2.12 × 102
Mean 5.19 × 107 * 6.43 × 106 * 5.51 × 106
SD 6.27 × 107 1.11 × 107 4.61 × 106
  1. LAMP – cells/g – bacterial cell number (log10 CFU equivalent) determined by LAMP
  2. LAMP – GE/g – genome equivalents of E. hirae per g of heart sample calculated by LAMP
  3. CFU – total viable E. hirae counts per g determined on selective agar after 24 h incubation
  4. *statistical significance (p = 0.028) between bacterial cell number determined by LAMP and CFU (LAMP – cells/g vs. CFU counting)
  5. The bacterial number of and genome equivalents were determined in 1 g of heart samples by LAMP and conventional plate-counting method