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Table 2 Detection of E. hirae in hearts of chickens representing different disease outbreaks

From: The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

No. of affected heart

LAMP – cells/g

CFU counting

LAMP – GE/g

H3

1.74 × 108

4.0 × 106

1.84 × 107

H7

1.13 × 108

5.0 × 106

1.2 × 107

H2

8.81 × 107

3.53 × 107

9.35 × 106

H4

6.91 × 107

2.0 × 106

7.34 × 106

H5

1.86 × 107

8.0 × 106

1.97 × 106

H1

4.84 × 106

3.3 × 106

5.14 × 105

H9

6.22 × 104

7.67 × 102

6.6 × 103

H6

9.36 × 103

2.33 × 105

9.94 × 102

H8

1.99 × 103

1.0 × 104

2.12 × 102

Mean

5.19 × 107 *

6.43 × 106 *

5.51 × 106

SD

6.27 × 107

1.11 × 107

4.61 × 106

  1. LAMP – cells/g – bacterial cell number (log10 CFU equivalent) determined by LAMP
  2. LAMP – GE/g – genome equivalents of E. hirae per g of heart sample calculated by LAMP
  3. CFU – total viable E. hirae counts per g determined on selective agar after 24 h incubation
  4. *statistical significance (p = 0.028) between bacterial cell number determined by LAMP and CFU (LAMP – cells/g vs. CFU counting)
  5. The bacterial number of and genome equivalents were determined in 1 g of heart samples by LAMP and conventional plate-counting method
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