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Table 1 Sequences of LAMP primers for detecting Enterococcus hirae (target sodA gene)

From: The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

Primers name

Sequence (5′-3′)

Base pair length

F3 (forward outer primer)

CCTACAGATATCAAGACTGCTG

22

B3 (backward outer primer)

GCTGTTGAAGTGATCGCTA

19

FIP (F1c + F2; forward inner primer)

ACCAGCATTTGGTGCCATGAGTAATAATGGTGGCGGACAT

40 (20 F1c, 20 F2)

BIP (B1c + B2; backward inner primer)

CGAACCAACTGGTGCAATTAAAGAAAATTCTTCCTTAAATGTTGCAAAATC

51 (25 B1c, 26 B2)

LoopF (loop forward primer)

TTCCAGAAGAAAGAATGGTTTGC

23

LoopB (loop reverse primer)

GCGATTGATGAAACCTTTGGT

21

  1. FIP consists of the F1c and F2 sequences; BIP consists of the B1c and B2 sequences. F1c and B1c were shown as underlined nucleotide sequences
  2. Amplicon size amplified with the outer primers F3/B3 is 248 bp (Novazym Poland s.c., Poznań)