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Fig. 7 | BMC Microbiology

Fig. 7

From: The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

Fig. 7

Melting curves generated after LAMP assay using (a) DNA of bacterial strains isolated from heart tissue samples and (b) DNA isolated directly from affected heart tissue samples of broiler chickens. Agarose gel electrophoresis of the LAMP products amplified from DNA of (c) bacterial strains isolated from heart tissue and (d) heart tissue samples of broiler chickens. Pos – positive control (E. hirae ATCC 10541), NTC - Non Template Control, Neg-Negative control, M-100-bp DNA ladder (SM0323, Thermo Fisher Scientific Inc., USA), c) Lane 4–19: bacterial isolates 1–16. d) Lane 4–12: 9 heart samples

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