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Fig. 5 | BMC Microbiology

Fig. 5

From: The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

Fig. 5

Agarose gel electrophoresis after (A) LAMP, and (B) PCR assay with F3/B3 LAMP primers and (C) species-specific primers using 10-fold dilutions of DNA E. hirae ATCC 10541 as a sensitivity indicator. a) Neg-Negative control, M-100-bp DNA ladder, Lane 3–11: LAMP assay products using serial dilutions b) Neg-Negative control, M-50-bp DNA ladder (SM0373,Thermo Fisher Scientific Inc., USA), Lane 3–11: PCR results using serial dilutions and F3/B3 LAMP primers (product length 248 bp) c) Neg-Negative control, M-50-bp DNA ladder, Lane 3–11: PCR results using serial dilutions and species-specific primers (product length 187 bp)

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BMC Microbiology

ISSN: 1471-2180

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