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Table 3 Sequences of forward (fd) and reverse primers (rev) used to amplify ompA, fadL, and carlo1 genes

From: Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice

Target gene NCBI accession Primer Sequence (5′-3′)a
ompA WP_018357032.1 OmpA_fd GAT CAG CCC GGG AAA
AAA ACT GCA ATC GCA TT
OmpA_rev GAT CAG CTC GAG TTT
AGA ACC ATT AAC TGC GA
fadL WP_018355328.1 FadL_fd GAT CAG CCC GGG AAA
AAA TTT AAT CAA TCT TT
FadL_rev GAT CAG CTC GAG TTA
GAA ACG ATA ATT TAC AT
carlo1 WP_018356225.1 CARLO-1_fd GAT CAG CCC GGG ACA
TTG TCA TTA GCC TTA CTT G
CARLO-1_rev GAT CAG CTC GAG GCC
AGC ATT ATA GCT AAC AC
  1. aUnderlined restriction sites of XmaI (CCC GGG) and XhoI (CTCGAG) were used for cloning of amplifications products into pET21b_JF