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Table 1 Bacterial strains, plasmids, and primers used in this study

From: Characterization of enzymatic properties of two novel enzymes, 3,4-dihydroxyphenylacetate dioxygenase and 4-hydroxyphenylacetate 3-hydroxylase, from Sulfobacillus acidophilus TPY

Strains/plasmids/primers Relevant characteristics Reference/Source
Strains   
Sulfobacillus acidophilus TPY Gram-positive, acidophilic, moderately thermophilic, isolated from a hydrothermal vent in the Pacific Ocean (12.2′29″N, 104.2′01″W; water depth 3083 m) [28]
E. coli DH5α F, ∆(lacZYA-argF)U169, recA1, endA1, hsdR17(rk, mk+), supE44, thi-1, gyrA, relA1 Invitrogen
E. coli DH5α (pET-32a(+) -hpaB) E. coli DH5α harboring plasmid pET-32a(+)-hpaB, Apr This study
E. coli DH5α (pET-32a(+) -hpaC) E. coli DH5α harboring plasmid pET-32a(+)-hpaC, Apr This study
E. coli DH5α (pET-28a(+) –mhpB2) E. coli DH5α harboring plasmid pET-28a(+)–mhpB2, Apr This study
E. coli BL21 (DE3) F, ompT, hsdS(rBB mB), gal, dcm(DE3) Invitrogen
E. coli BL21 (DE3) (pET-28a(+)-mhpB2) E. coli BL21 (DE3) harboring plasmid pET-28a(+)-mhpB2, Apr This study
E. coli BL21 (DE3) (pET-32a(+)-hpaB) E. coli BL21 (DE3) harboring plasmid pET-32a(+)-hpaB, Apr This study
E. coli BL21 (DE3) (pET-32a(+)-hpaC) E. coli BL21 (DE3) harboring plasmid pET-32a(+)-hpaC, Apr This study
Plasmids   
pET-28a(+) Expression vector, Kmr, His-tag Takara
pET-32a(+) Expression vector, Apr, His-tag Takara
pET-32a(+) -hpaB Plasmid pET-32a(+) harboring hpaB gene, Apr, His-tag This study
pET-32a(+) -hpaC Plasmid pET-32a(+) harboring hpaC gene, Apr, His-tag This study
pET-28a(+)-mhpB2 Plasmid pET-28a(+) harboring mhpB2 gene, Kmr, His-tag This study
Primers Sequence (5′-3′) Restriction site
hpaB F: 5′-AATGATATCATGGGGATTCGGACCGGCGAA-3′ EcoRV
R: 5′-GGAAAGCTTTTACGACCGCGCCTCCTTTGT-3′ HindIII
hpaC F: 5′-AATGATATCATGGGGATTCGGACCGGCGAA-3′ EcoRV
R: 5′- GGAAAGCTTCTACCGTTCCAGTAACAAA-3′ HindIII
mhpB2 F: 5′-GGAATTCATGGGCGAAGCATTTAATAT-3′ EcoRI
R: 5′-GGAAAGCTTTTAATCGTCAAGAAACGCCGG-3′ HindIII
  1. EcoRV and HindIII recognition sites are underlined