- Research article
- Open Access
Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling
BMC Microbiology volume 19, Article number: 49 (2019)
Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. The 20 soil and sand samples were collected from different locations in the desert and the actinobacterial strains were selectively isolated. The isolated strains were identified using a polyphasic taxonomic approach including morphological, biochemical, physiological characterization, scanning electron microscopy (SEM) and by 16S rRNA gene sequencing.
A total of 110 desert actinobacterial strains were recovered, which were found to be belonging to 3 different families of the order Actinomycetales, including the family Streptomycetaceae, family Pseudonocardiaceae and the family Micrococcaceae. The most frequently isolated genus was Streptomyces along with the genera Pseudonocardia and Arthrobacter. The isolated strains exhibited promising antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) with zone of inhibition in the range of 9–32 mm in antimicrobial screening assays. The chemical profiling by thin layer chromatography, HPLC-UV/Vis and LC-MS analysis depicted the presence of different structural classes of antibiotics.
The study revealed that Cholistan desert harbors immense actinobacterial diversity and most of the strains produce structurally diverse bioactive secondary metabolites, which are a promising source of novel antimicrobial drug candidates.
The actinomycetes thriving in harsh environments have the capability to endure the extreme temperature, drought conditions and to produce very unique antibacterial compounds. The desert soils are rich and untapped source of novel strains of these extraordinary species. There is a need to explore and screen these niches for the diversity of these prolific sources of antibiotics to combat the alarming situation of antimicrobial resistance. The Cholistan desert in Pakistan is a unique and unexplored ecological system covering the expanse of 26,300 km2 in the south of district Bahawalpur in the Punjab. It extends to the Thar desert in Sindh between longitudes 69°52′ to 73°24′ E and latitudes 28°42′ to 29°25′N (Fig. 1), present at an altitude of 89 m above sea level . With reference to the climate, it is categorized as harsh, sub-tropical region with scarcity of precipitation and long dearth season, low relative humidity, with high rate of evaporation [2, 3]. The soil is very poor in organic matter and it may possess unique actinomycetes with unusual antibacterial activity. Although the actinomycetes are present in various environments like soil, fresh water, marines, in the plant tissues etc. but those present in unexplored habitats may have some unique characteristics to live in such habitats and to produce unique secondary metabolites . These are abundantly present in soil of different types, but the arid habitats like deserts are now becoming the target ecosystems for their search because of the uniqueness of environmental conditions.
Because of the ease of access to antibiotics, the misuse of these drugs has been increased, as a result we are lacking in having a perfect antibiotic which can eradicate the pathogens without causing harmful side effects . New antibiotics are required to fight against the counterfeiting pathogens that have developed resistance against existing antibiotics . Methicillin resistant Staphylococcus aureus (MRSA) has been designated as one of the major hazardous pathogens associated with the development of antimicrobial resistance (AMR). The advent of AMR in S. aureus is widely recorded in Pakistan and around the globe and the species has proven particularly skilled at evolving resistance in the face of new antibiotic challenges. Therefore, the search for unrevealed species is critically needed to address this emerging problem and there is a recommendation to explore the untapped environments like deserts, caves etc .The exploration of new species of actinobacteria will likely give a chance to discover potentially new structural and beneficial secondary metabolites. The actinomycetes isolated from deserts have the capability to survive the harsh conditions of weather like high pH of the soil and salinity, and they possess unusual gene clusters for the production of unique and potent antibacterial compounds .
Different techniques are being used to identify these unique bacterial species and the compounds produced by them like microscopic analysis . Microscopic studies especially scanning electron microscopic (SEM) examination of different species of actinomycetes provides useful information about their ultra-structures. The spores are considered to be the electron dense structures and the examination of their surfaces and arrangement in the form of chains and clusters has been studied by SEM. The spore arrangement is considered as an important criterion for the determination of the taxonomic position of actinomycetes in different groups. The use of SEM is increasing for the classification of actinomycetes because of its higher magnification and great focal strength . Now, very high quality polycarbonate filters are available with different pore sizes for the collection of viruses and bacteria which are suitable for their surface observation using SEM .
A little information is present regarding the actinomycetes isolated from harsh and arid ecosystems, which are considered as the most bountiful environments for the discovery of new bacteria and compounds . In our previous report , we reported the identification and antimicrobial activity of some selected strains from this ecosystem against methicillin resistant Staphylococcus aureus (MRSA), with major emphasis on the identification and validation of MRSA strains from clinical samples. This study reports a detailed account of the search and screening of the unique and extreme ecological niche; the Cholistan desert, Pakistan for the culture-able spore forming actinobacterial diversity, along with the in depth chemical profiling of the bioactive compounds produced by them. We believe that this ecosystem has not been studied for the detailed actinobacterial diversity, so this seems to be the first comprehensive report on culture-able spore forming actinobacterial diversity of the Cholistan desert Pakistan.
The 20 soil samples were collected in polythene bags from various sites in Cholistan desert Pakistan. The samples were obtained at least 1–2 km distance from each other. These samples were then processed first by applying different physical and chemical treatments (Heat treatment was given at 50–55 °C for 2–16 h and chemical treatment was given by mixing the soil sample with CaCO3 in the ratio 10:1 (w/w) followed by incubation at room temperature for 7–9 days in humid environment) by adopting the methods narrated by Hayakawa and Nonomura  for the selection of actinobacteria.
Isolation of Actinobacteria
For the isolation of actinobacterial strains, serial dilution method was adopted for each sample. Two different isolation media were used according to the guidelines of International Streptomyces Project (ISP) for the isolation of variety of strains: glycerol-casein-KNO3 agar (Sigma) and actinomycete isolation agar (media recipes are included in additional file 1) were prepared and sterilized by autoclaving. To prevent or suppress the fungal growth, cycloheximide (20 mg/L) was added in the media after autoclaving. The serial dilutions of the samples were prepared and 50 μl of the dilutions 10− 2 and 10− 3 were spreaded on isolation media, followed by incubation at 28 °C for 7 to 15 days for both of the media. The prospective actinobacterial colonies were chosen from media plates and were streaked on the enriched medium i.e. Yeast extract-malt extract (GYM) agar (Sigma)  and then purified by repeated streaking on GYM agar. The pure cultures were preserved as glycerol stocks at − 80 °C in the microbial storage facility at the Department of Microbiology and Molecular Genetics (MMG), University of the Punjab (a redundant copy of the strains has also been stored in the collaborating laboratory at the Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky). The strains were initially screened for the antimicrobial activity against MRSA by the agar plug method or the so called solid media bioassay  and active strains in whole collection were detected (Additional file 2; Table S1).
Taxonomic characterization of the Actinobacterial strains
A polyphasic taxonomic approach was adopted for the identification of the isolated strains. All of the strains were studied for morphological, physiological and biochemical characteristics, along with the 16S rRNA gene sequencing of the 30 representative strains. The morphological characterization was performed with the pure and individual strains by following the methods of Bensultana et al. . The features observed, included colony morphology, color of aerial and substrate mycelia and pigmentation. The strains were probed for biochemical features including the production of melanin, utilization of different sugars, organic acid formation, utilization of oxalate and organic acid, hydrolysis of esculin & urea.
For genetic characterization, the DNA of actinobacterial strains was extracted from mycelial part, using tissue genomic DNA extraction kit (FavorPrep™, Cat# FATGK001–1). The 16S rRNA gene sequence of these DNA samples were amplified by PCR amplification, using universal primers (27f: AGAGTTTGATCCTGGCTCAG) and (1522r: AAGGAGGTGATCCARCCGCA) . The PCR reaction mixture included 5 μl of DNA sample, 2 μl of each primer with working concentration of 10 pmol and 25 μl of 2 X PCR master mixes (THERMO-scientific DreamTaq Green Master Mix). The reaction conditions were as follows: 95 °C for 1 min, 30 cycles of 95 °C for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 30 s followed by final extension for 5 min at 72 °C . Amplified products were purified by employing the protocol described in gel purification kit (FavorPrep™, Cat# FAGPK001–1), then sequenced on an automated sequencer. The sequenced data obtained was BLAST analyzed at the NCBI  as well as at EzBioCloud database , for determining the similarity with type strains. The analyzed sequences of each strain were deposited to the NCBI GenBank to get the accession numbers. The evolutionary history was inferred by constructing phylogenetic tree by Neighbor-Joining method using 1000 replicates bootstrap value [21, 22]. The evolutionary distances were computed using the Maximum Composite Likelihood method  and are in the units of the number of base substitutions per site. Evolutionary analyses were conducted in MEGA6 .
Scanning Electron microscopy (SEM)
The ultra-structure of mycelium and arrangement of spores of representative desert actinobacterial strains was observed by using scanning electron microscopy following the procedure described by Cavaletti et al. . A small piece of GYM agar from the purified culture plate of actinobacteria was trimmed with a sterilized scalpel and fixed with a solution of cacodylate buffer having 5% formaldehyde and 2% glutaraldehyde (0.1 M cacodylate, 0.01 M CaCl2, 0.01 M MgCl2, 0.09 M sucrose; pH 6.9) for 3 h on ice and washed with the cacodylate buffer. Dehydration of the samples was done by using different grades of acetone (10, 30, 50, 70, 90, and 100% acetone) and each step was performed on ice block for 30 min. After that the samples were dried by using liquid CO2 and covered with an approximately 10 nm thick gold film by spattered coating. These samples were then observed under a range of magnifications (5.00 kx, 10.0 kx, 15.0 kx, 20.0 kx, 25.0 kx, 40.0 kx and 50.0 kx) with acceleration voltage 5.0 kV, 7.0 kV and 10.0 kV in VEGA3 (TESCAN) Scanning Electron Microscope by using SE (secondary electron) detector.
Preparation of Methanolic extracts of desert Actinobacteria
The desert actinobacterial isolates were grown on lab scale as shaking flask cultures (250–500 ml GYM broth) and the crude extracts were obtained by solvent-solvent extraction using a separating funnel. The equal volumes of culture broth and ethyl acetate were mixed in the separating funnel and shaken for about 15 min, after which the mixture was allowed to settle down. After the two distinct layers appeared, the upper organic layer was retrieved carefully and the procedure was repeated thrice. The ethyl acetate was then evaporated on rotary vacuum evaporator (Heidolph Laborota 4000 efficient, Type# D-91126 Schwabach) and the extract was dissolved in analytical grade methanol.
Determination of antibacterial activity against MRSA
The antimicrobial activity of desert actinobacterial strains was determined at two levels, initially all the isolated 110 strains were screened by agar plug method against one MRSA strain and active strains were selected. Later the active strains were further screened by well diffusion methods against the 10 MRSA strains. For this purpose ten strains of MRSA were collected from a diagnostic laboratory (Citi Lab, Lahore). These strains were confirmed as MRSA by performing different biochemical tests (catalase and DNase), through disc diffusion antibiotic assay using a panel of 10 antibiotics discs including methicillin (10 μg), oxacillin (1 μg), cefoxitin (30 μg), ampicillin (10 μg), amikacin (30 μg), amoxicillin (25 μg), cephalexin (30 μg), cefoperazone (75 μg), ciprofloxacin (5 μg) and piperacillin (100 μg) according to the CLSI standards . The validation of these strains was also established genetically by mec-A gene and 16S rRNA gene sequencing  (GenBank Accession numbers: KU662352-KU662355, KR862284, KR862285, KR862287- KR862289, KR862291).
The discs of agar (5 mm) were cut from the pure culture plates of actinobacterial strains and placed on the MH (Muller Hinton agar) plates swabbed with MRSA isolate and zones of inhibition were recorded after overnight incubation . The well diffusion method was performed by loading 60 μl of methanolic extracts of actinobacterial isolates in the wells (5 mm diameter) made in MH agar swabbed with MRSA isolate. The zones of inhibition were measured around the wells after overnight incubation .
Chemical profiling of the Actinobacterial strains
The methanolic extracts of the bioactive actinobacterial strains were screened for their chemical profile by employing three chromatographic techniques: thin layer chromatography, high performance liquid chromatography (HPLC) and LC-MS.
Thin layer chromatography
Crude extracts of actinobacterial strains were tested on TLC plates (TLC silica gel 60 F254, Merck, Germany) to assess the nature of secondary metabolites produced by them. Each of the extracts was superimposed onto two TLC plates by repeated spotting using a small capillary. The loaded plate was run in 10% methanol/dichloromethane solvent system and visualized in UV light (two wavelengths were used i.e., 254 nm and 366 nm) using a UV lamp (CAMAG). After the development, one plate was stained with anisaldehyde/H2SO4 spraying reagent (anisaldehyde, methanol, H2SO4, acetic acid) while the second plate was sprayed by Ehrlich’s reagent (HCl 37%, 4-dimethylamino benzaldehyde, methanol) in order to determine different colored bands of the compounds in the extracts.
High performance liquid chromatography (HPLC-UV/Vis) analysis
HPLC-UV/Vis analysis of crude extracts of actinobacterial strains was carried out on clarity chromatography software in Sykum® S1122 delivery system. The separation of the components of secondary metabolites was carried out by using RPC18 column (ThermoHypersil-Keystone, 250 × 4.6 mm, particle size: 5 μm) which act as a stationary phase. The mobile phase was methanol and water (9:1) while the rate of flow was set at 1 ml/min. Various peaks were observed in the UV chromatograms of the samples which were compared later with the UV chromatographic data of secondary metabolites to interpret the type of compounds produced by the actinobacterial strains.
High performance liquid chromatography- mass spectrometry (HPLC-MS) analysis
HPLC-MS analysis of the most active actinobacterial extracts was performed on the Agilent 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent 1200 Series Quaternary LC system and an Eclipse XDB-C18 column (150 × 4.6 mm, 5 μm; solvent A: H2O/0.1% formic acid, solvent B: CH3CN/0.1% formic acid; flow rate: 0.4 ml min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–35 min, 10% B). The molecular masses on each of the ion peak were calculated in all the extracts and the data was analysed by comparison with the already reported Streptomyces compounds in the AntiBase .
Taxonomic characteristics of the selected Actinobacterial strains
A total of 110 actinobacterial strains were recovered from the desert soil and sand samples. Most of the strains produced hard, embedded and rough textured colonies with different sizes and regular or irregular margins. A variety of colors of aerial mycelia were observed in different strains including white, yellowish white, yellow, purple, orange and pink etc, similarly the substrate mycelia displayed the colors including white, yellowish, brown, light pink, purple and orange etc (Table 1, Additional file 3: Table S2, Additional file 4: Figure S1. The diffused pigments were observed only in 3 strains including, AFD1 (brown), AFD15 (light brown) and in AFD26 (dark yellow) (Table 1, Additional file 3: Table S2, Additional file 4; Figure S1).
In case of the testing of different sugars as sole source of carbon, the glucose and mannose were utilized by all of the strains except the strain, AFD23. Similarly fructose was used as carbon source by most of the strains, except four strains including AFD6, AFD14, AFD20 and AFD26 (Table 2). Sixteen of the strains exhibited the tyrosinase activity and production of melanin. Most of the strains showed hydrolysis of urea and esculin, utilization of organic acids and oxalate etc. Similarly most of the strains were found positive in case of organic acid formation and lecithovitellin reaction (Table 2, Additional file 3: Table S2).
In scanning electron microscopy using VEGA3 (TESCAN), different magnifications (5.0 kx, 10.0 kx, 20.0 kx, 25.0 kx, 40.0 kx, and 50.0 kx) revealed ultra-fine structure of the cells and arrangements of spores of actinobacterial strains, the best results were obtained by using 40.0 kx magnification (Fig. 2). As it can be seen in Fig. 2, the arrangement of spores in the form of chains is clear for all the isolates. In case of the strain AFD1, best results were obtained at higher magnification i.e. 25 kx. The spores are grouped in the form of chains with lateral depressions (Fig. 2a and b). In case of the strain AFD7 the spores can be seen entangled with each other to form the aerial mycelium on the agar surface. At higher magnification of the SEM (25 kx) the typical arrangement of the spores i.e. Retinaculiaperti  of this strain is clear (Fig. 2c and d). Some of the strains have unique pattern of spores arrangement as in case of strain AFD8 (Fig. 2e), showing a sheath of cells while in the Fig. 2f the spores are emerging from the cells like spines. Another unique arrangement was shown by the strain AFD16 (Fig. 2g, h) in which the spores are entangled to form an interlocking pattern. In case of the strain AFD19 long and mature spores can be seen as 10–50 spores per spore chain (Fig. 2i, j). In some strains, spiny surfaced spores can be seen with central depressions like in the case of strain AFD26 (Fig. 2k, l). The morphological (colony characteristics), microscopic (SEM), biochemical and physiological characteristics suggested that most of the isolated strains are the members of genus Streptomyces on comparison of the features with those of reported in the literature [29, 30].
The 16S rRNA gene sequences and BLAST analysis at EzBioCloud database  of the 29 representative strains showed %similarity with different actinobacterial type strains. In 25 of the cases %similarity in the range of 99 to 100% was observed with different species of the genus Streptomyces (Table 3). However 4 strains exhibited %similarity between 98 to 99%, such as strain AFD6 exhibited 98.68% similarity with the type strain Streptomyces fradiae (MIFZ01000280), the strains AFD23 and AFD26 exhibited 98.27 and 98.94% respectively, the %similarity with the type strain Streptomyces albaduncus (AY999757), the strain AFD29 showed 98.97% similarity with type strain Streptomyces europaeiscabiei (NR042790). While 1 strain AFD13 exhibited 97.02% similarity with the type strain Streptomyces roseofulvus (AB184327) (Table 3). Two of the strains exhibited %similarity with the type strains of genera other than Streptomyces, including the strain AFD18 showed 99.65% similarity with the type strain Saccharothrix xinjiangensis (AB381939), while the strain AFD22 showed 99.89% similarity with the type strain Arthrobacter ANPE_s (ANPE02000028) (Table 3).
The neighbor joining phylogenetic tree constructed based on the 16S rRNA gene sequence data of the 29 strains, shows the phylogenetic relation of the strain with each other. The optimal tree with the sum of branch length = 32.44194265 is shown in Fig. 3. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method  and are in the units of the number of base substitutions per site. The analysis involved 49 nucleotide sequences, for which the NCBI GenBank accession numbers are given in parenthesis. Codon positions included were 1st + 2nd + 3rd + Noncoding. All positions containing gaps and missing data were eliminated. There were a total of 254 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 . The numbers at the nodes indicate the levels of bootstrap support based on 1000 replicates. The isolated strains showed phylogenetic relationships with each other and clustered in four major clads, showing various Streptomyces species with common ancestry. The strains made pairwise operational taxonomic units (OTUs) at species level with each other and with the closest match or type strains found by BLAST analysis via EzTaxon server (Fig. 3).
Antibacterial activity of the selected Actinobacteria against MRSA
The actinobacterial strains displayed prolific antibacterial activity against MRSA isolates. In initial screening by agar plug method among the 110 isolated strains 30 strains showed growth inhibition of the MRSA test strain (Additional file 2: Table S1). The 30 active strains were further investigated for their inhibitory activity by well diffusion assay against a panel of 10 MRSA strains. In this secondary screening the most active strain was Streptomyces pseudogriseolus AFD2 which exhibited the maximum zone of inhibition up to 32 mm against the MRSA strain A11. The strain AFD4 displayed maximum zone of inhibition (18 mm) against MRSA strain A5. In the case of strain AFD7, maximum zone of inhibition was recorded to be 24 mm. The strain AFD8 exhibited maximum zone of inhibition against MRSA strain A1 and A5 i.e., 17 mm. The strain AFD9 showed maximum zone of inhibition 21 mm against MRSA strain A2. The strain AFD10 also exhibited very potent antibacterial activity with zone of inhibition of 20 mm against MRSA strains A2, A6 and A14. The strain AFD14 showed maximum zone of inhibition i.e., 23 mm against MRSA strain A7. The strain AFD15 exhibited maximum zone of inhibition against MRSA strain A9 i.e., 21 mm. Among other desert actinobacterial strains, another highly active strain was AFD16; it exhibited zone of inhibition up to 20 mm against MRSA strains A1, A5, A6 and A9. In the case of strain AFD20 maximum zone of inhibition recorded was 27 mm against MRSA strain A8 while in case of strain AFD21 maximum zone of inhibition recorded was 19 mm against MRSA strains A1, A5, A6 and A9. The strain AFD22 showed 26 mm zone of inhibition against MRSA isolates A1 and A14 (Table 4, Additional file 5: Figures S2A, S2B, S2C and S2D).
Chemical profiling of the Methanolic extracts of Cholistan Desert Actinobacteria
In TLC analysis, the UV visible bands were observed at 254 nm and 366 nm wave length. The methanolic extracts of the strains AFD7, AFD9, AFD10, AFD20, AFD22, AFD24 and AFD26 developed some distinct bands when viewing in UV light (Additional file 5: Figure S2E, S2F). Different colored bands were observed in the extracts of the strains AFD9, AFD10, AFD20, AFD22 and AFD24 after treatment with anisaldehyde reagent. The spots of different colors like violet, blue, green, yellow and orange were seen after heating which expressed the presence of different structural classes of secondary metabolites like quinolones and actinomycins etc .
The HPLC-UV analysis of the crude extracts of the active strains exhibited varied peaks. For example, the crude extract of the actinobacterial strain AFD2 produced 3 prominent and many minor peaks, the highest was observed at 9.323 min, then at 2.876 min and 4.144 min (Additional file 6: Figure S3). The crude extract of another active strain AFD7 showed 6 peaks, the major was at retention time 4.113 min (Additional file 7: Figure S7). Besides these the crude extracts of other strains like AFD4, AFD8, AFD10, AFD12 and AFD26 also exhibited variety of peaks at different retention times (tR) with varied peak areas (Additional files 8: Figure S5, S9, S11, S13, S15 and S17, Additional files 9, 10, 11, 12 and 13).
The LC-MS analysis of the extracts of most active actinobacterial strains displayed an exciting diversity of the components with antibacterial activity. As shown in Table 5, the total ion chromatogram of strain AFD2 showed various peaks at different retention times (tR) the most prominent of which was observed at tR 6.38 min and the molecular mass calculated for this component was 112 Da. The comparison of the chemical profile of this component with the compounds in AntiBase gave the closest match with Emimycin, 3, 5-Dimethyl-3-oxol-2-on, and Uracil. Similarly the components at tR 11.03 and 12.57 min in this extract gave closest match with various other compounds in AntiBase (Table 5, Fig. 4 and Additional files 14: Figure S4 and S19, Additional file 15).The total ion chromatogram of strain AFD4 showed varied peaks at different retention times (tR), such as a peak at 12.60 min with molecular mass 136 Da. The chemical profile of this component was then compared with the compounds in database and found the closest match with Pyrrole-3-yl-2-propenamide, Octalactin C and Anthranilamide. Likewise the components at tR 10.23 and 12.22 min also showed close matches with the various compounds in the AntiBase (Table 5, Fig. 4 and Additional files 16: Figure S6, S19, Additional file 15). The total ion chromatogram of the strain AFD7 showed peaks at different retention times such as at tR 3.83 min, molecular mass for which was calculated as 152 Da. The chemical profile of this component was compared with the compounds in AntiBase and the closest matches were observed with Xanthine, Guanazoloand Orsellin-aldehyde. The other components in this extract at other tR 4.50 min, 5.77 min, 7.10 min, 10.24 min and 11.07 min were also compared in the same manner with the compounds in database and the prospective compounds were suggested (Table 5, Fig. 4 and Additional file 17: Figure S8, S19, Additional file 15).
The total ion chromatogram of strain AFD8 showed various peaks at different retention times, the most prominent of which was observed at 13.42 min, and the molecular mass for this was calculated as 256 Da. The chemical profile of this component was then compared in the database and found the closest match with Phenacein, Isobutyryl pyrrothine and Albonoursin. In the same manner the components appeared at other retention times 9.07 min, 14.94 min and 15.58 min were also compared with the AntiBase (Table 5, Additional file 18: Figure S10, S19, Additional file 15).The total ion chromatogram of strain AFD10 exhibited several peaks at different retention time (tR) ranging from 9.90–19.42 min and the molecular masses calculated for some of the selected peaks were assigned as 210, 136, 400 and 474 Da. The chemical profiles of all of these components were also compared with the compounds in AntiBase and found their close matches e.g. the component with molecular mass 400 Da showed the closest match with Daunomycinol, Furaquinocin G, and Daidzein-7-α-L-rhamnoside (Table 5, Additional file 19: Figure S12, S20, Additional file 20). The crude extract of strain AFD12 also exhibited good LC-MS results as it displayed significant peaks at 4.36–16.06 min, with molecular masses range from 111 to 206 Da. The chemical profiles of all of these components were compared with the database and found their close matches, e.g. the component detected at tR 4.36 min with molecular mass 159 Da showed a close match with 4-(Hydroxymethyl) quinolone and Quinazolinamine (Table 5, Additional files 21: Figure S14, S20, Additional file 20). While strain AFD14, did not show clear mass based on the HPLC-MS analysis (Additional file 22: Figure S16), it displayed an interesting HPLC-UV/Vis profile (Additional file 12: Figure S15). Finally, the crude extract of strain AFD26 exhibited various peaks at retention times (tR)/molecular masses 6.32 min/112 Da, 6.38 min/163 Da, 9.49 min/163 Da, 10.85 min/163 Da, 12.23 min/316 Da and 17.43 min/390 Da. The chemical profile of all of these components were compared with the data in AntiBase and found the closest matches with reported compounds. For example the component with molecular mass 316 Da showed the close match with Boxazomycin A, 17927-D and Isobutyroxymethyl cyclohex-1(6)-ene-2, 3, 4, 5-tetrol-2-isobutyrate (Table 5, Additional files 23: Figure S18, S20, Additional file 20).
The need for new bioactive metabolites from untapped environments like deserts is mainly stressed due to the drastic consequences and vivacious nature of antibiotic resistance in pathogens. The Cholistan desert of Pakistan is one of those environments which have not been explored yet for the detailed actinobacterial diversity and the search for antimicrobial producing organisms. So, it is anticipated that it may harbor unique and novel species of actinobacteria which can produce new antimicrobial drugs. There is a great need for novel and potential compounds discovery, with their pharmaceutical, industrial or agricultural applications . The actinomycetes are the most authentic sources for new antibiotics because of their varied chemical nature, wide taxonomic scope and environmental distribution. Harsh conditions like dryness, extreme temperatures and alkalinity directed to the isolation of very resistant actinomycetes from untapped environments like deserts, caves etc. Different useful strategies are required to exploit these habitats for the discovery of stress tolerant bacterial species .
In the present study 110 actinobacterial strains were isolated from the Cholistan desert. The polyphasic taxonomic characterization including morphological, microscopic, biochemical and physiological characteristics and 16S rRNA gene sequence analysis suggested that these isolates belong to different actinobacterial genera and species. In biochemical characterization it was found that these were able to produce melanin pigment which may be an avoidance strategy to the desiccation and intense radiation, as described by many researchers like Bull and Asenjo . They reported that the actinobacteria living in extreme conditions can be metabolically functional and they may not be considered as dormant. Another very important feature for the identification of actinomycetes especially Streptomyces is the determination of utilization of different sugars as a source of carbon. For this purpose these strains were tested for their ability to utilize 8 different sugars (D-fructose, D-glucose, D-galactose, L-arabinose, D-mannitol, sucrose, inositol and mannose). It was found that most of the strains used mainly the glucose as the carbon source, however fructose and sucrose were the next sugars which were used most frequently, these results are comparable with the findings of Aftab et al. .
The actinobacterial spores have the capability to germinate in very low available water environments and it enables them to adapt to drought conditions. Our major focus has been on the spore forming actinobacterial diversity, so scanning electron microscopic (SEM) analysis of the aerial spores and substrate mycelium of some of the representative strains showed extensive substrate mycelium and interlocking patterns of the cells, which might be due to the high temperature, high salinity, and radiations effect [10, 35]. A variety of shapes of spores were observed using different magnifications (5 kx, 10 kx, 15 kx, 20 kx, 25 kx, 40 kx and 50 kx), which were arranged in the form of chains and clusters showing various stages of spore development (Fig. 2). The comparison of the spores arrangement and shapes with the descriptions given in Bergey’s manual of systematic bacteriology [29, 30] indicated the major resemblance of these strains with different Streptomyces species.
The 16S rRNA gene sequencing and BLAST search via EzTaxon server and subsequent phylogenetic analysis gave the in-depth insights into the actinobacterial diversity in this extreme ecological niche. Mainly the strains belonged to three different families of the order Actinomycetales, including the family Streptomycetaceae, the family Pseudonocardiaceae and the family Micrococcaceae. In most of the cases strains shared > 99% genetic similarity with the Streptomyces type strains, while 5 of the strains including AFD6, AFD13, AFD23, AFD26 and AFD29 showed < 99% similarity, usually in the range of 97–98% (Fig. 3). This shows that these 5 strains could be different taxon or new Streptomyces species; however the complete 16S rRNA gene sequences and other relevant genetic approaches such as DNA-DNA hybridization with the type strain etc can further unveil their exact taxonomic status. Okoro et al.  reported that majority of the isolates from the soil of Atacama Desert located in south America belonged to the genera Amycolatopsis, Lechevalieria and Streptomyces.
The second major goal in this study was to detect and obtain the actinobacterial strains which exhibit potent activity against MRSA (methicillin resistant Staphylococcus aureus). MRSA has been designated as the superbug due to its resistance to almost all the known antibiotic classes. In this scenario activity against MRSA is extremely needed to search the new antimicrobial drug candidates. The actinobacterial strains from this unique ecological environment exhibit remarkable activity against MRSA test strains. Among the 110 strains, in initial screens by agar plug method 30 strains were detected to be active against MRSA. While, further screening through well diffusion method revealed the potent inhibitory activity of these strains against all the MRSA strains. Especially, the strain AFD2 Streptomyces pseudogriseolus showed 32 mm zone of inhibition against MRSA. In general, the zone of inhibition in the range of 9–32 mm was observed which is comparable to the findings of Nithya et al. , who reported the inhibition of MRSA by desert actinomycetes isolated from Saudi Arabian desert.
The chemical profiling of the crude extracts of these actinobacterial strains showed that they produce compounds belonging to different structural classes, as indicated by colored bands on TLC after staining with anisaldehyde/H2SO4. For instance, the development of yellow spots indicated the production of actinomycin like compounds, pink spots indicated quinolone like compounds, blue spots were indicative of peptide like fractions, however the peptides are needed to confirm by staining with iodine vapors etc. The greenish spots are usually shown by allylic alcohols, red spots for amines, aldehydes, ketones, carbohydrates and esters (alkyl phthalates) and violet spots for phenols etc. The HPLC/UV chromatograms exhibited a comparative account of the metabolic capability of the isolated actinobacterial strains, as varied number of peaks (up to 7–8 peaks) at different retention times (tR) were observed in the methanolic extracts. This shows that these actinobacterial strains are capable to produce variety of different compounds at the same time with different concentrations. The LC-MS analysis of the strains with prominent activity against MRSA indicated that these actinobacterial strains produce low molecular weight compounds usually < 500 Da. This shows that these active components can be potential antibiotic candidates because the higher molecular weight compounds usually show various cytotoxic effects in the subsequent drug development process. The metabolic profiles of the methanolic extracts of these actinobacterial strains are comparable with those reported by Taddei et al.  and Anwar et al. , where 5–8 major metabolites has been detected in the extract of one strain.
The comparison of the biological activity and chemical profile of the compounds produced by these strains with those of reported in AntiBase provides deeper insights in to the chemical nature/identity and structural diversity of these active compounds. The AntiBase  is a largest and most authentic database with more than 40,000 natural compounds produced by different microorganisms especially by Streptomyces. For instance, the strain AFD2 showed the production of a compound which had the close match with the antibiotic Emimycin and Triacsin. In the same manner, the strain AFD4 exhibited the production of different compounds with close matches in database with the antibiotics like 1-Methoxyphenazine and Pyrrole-3-yl-2-propenamide. Similarly, the strain AFD7 exhibited the production of various compounds with close matches with antibiotic compounds such as, Xanthine, Pentenomycin I and Fibrostatin B. The strain AFD8 exhibited the presence of different compounds which had the close matches with the compounds in the AntiBase e.g. o-Hydroxyphenylacetic acid and Phenacein. The strain AFD10 showed the production of different compounds which had the close match with the different antibiotic compounds in the database including Anthranilamide and 1-Methoxyphenazine. The strain AFD12 also exhibited the production of different active components which showed the closest matches with 4-(Hydroxymethyl) quinolone and Nikkomycin E. The strain AFD26 exhibited the production of various antibiotic components which had the close matches with Emimycin, Dihydroabikoviromycin and Boxazomycin A (Table 5). The results showed that the diverse spore forming actinobacterial strains are present in this harsh ecological niche and most of them are capable of producing variety of structurally diverse compounds.
The study revealed that the Cholistan desert in Pakistan harbors an immense and untapped diversity of culturable spore forming actinobacteria with valuable antimicrobial potential against MRSA. The most frequently isolated strains in this study belong to the family Streptomycetaceae, and 27 different species of genus Streptomyces were identified. The two strains were identified to be the members of different families, such as strain AFD18 Saccharothrix xinjiangensis belongs to the family Pseudonocardiaceae and the strain AFD22 Arthrobacter sp. belongs to the family Micrococcaceae. Our findings based on in-depth chemical profiling confirms that most of the strains from this extreme and harsh environment are capable of producing variety of low molecular weight secondary metabolites belonging to different antibiotics structural classes, which should be explored further for new antimicrobial drug candidates.
Basic Local Alignment Search Tool
Clinical and Laboratory Standards Institute
Geographic Information System
Glucose-yeast extract-malt extract
High Performance Liquid Chromatography-Ultraviolet/Visible
International Streptomyces Project
Liquid Chromatography- Mass Spectrometry
Molecular Evolutionary Genetics Analysis Version 6
Microbiology and Molecular Genetics
Methicillin Resistant Staphylococcus aureus
National Center for Biotechnology Information
Operational Taxonomic Units
Polymerase Chain Reaction
Ribosomal Ribonucleic Acid
- S. aureus :
Scanning Electron Microscopy
Thin Layer Chromatography
- t R :
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This work was supported by the grant from Higher Education Commission (HEC) Pakistan (Grant No. PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121). This work was also supported by National Institutes of Health grant R24 OD21479 (JST), The University of Kentucky, College of Pharmacy and the National Center for Advancing Translational Sciences (UL1TR001998 and UL1TR000117). The grants PIN: 112–31652-2BM1–172, and HEC-NRPU Project 2121, provided the support in terms of student’s stipend and experimental design, travel cost for sample collection, research supplies, reagents, DNA sequencing cost, data analysis and writing the paper. Grants R24 OD21479 (JST), UL1TR001998 and UL1TR000117 supported, in part, personnel and reagents associated with chemical screening, analytical methods and data analysis.
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Isolation media used for the Actinobacterial Diversity (PDF 279 kb)
Table S1. Primary screening of all the isolated desert actinobacterial strains against MRSA by agar plug method (PDF 201 kb)
Table S2. Microbiological and biochemical characteristics of Cholistan desert actinobacterial strains (PDF 309 kb)
Figure S1. Morphological appearance of selected Cholistan desert actinobacterial strains on GYM agar (A) strain AFD2 Streptomyces pseudogriseolus (B) strain AFD3 Streptomyces canarius (C) strain AFD6 Streptomyces fradiae (D) strain AFD13 Streptomyces roseofulvus (E) strain AFD16 Streptomyces pratensis (F) strain AFD10 Streptomyces puniceus (PDF 250 kb)
Figure S2. Antibacterial activity of the selected actinobacterial strains against MRSA isolates: (A, B) Zone of inhibition in agar plug method, (C, D) zone of inhibition in well method, (E) different bands of crude extracts of actinomycete isolates under UV at 366 nm (F) at 254 nm wavelength. (PDF 232 kb)
Figure S3. HPLC/UV analyses of AFD2 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 224 kb)
: Figure S7. HPLC/UV analyses of AFD7 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 246 kb)
Figure S5. HPLC/UV analyses of AFD4 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 59 kb)
: Figure S9. HPLC/UV analyses of AFD8 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 63 kb)
Figure S11. HPLC/UV analyses of AFD10 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 346 kb)
Figure S13. HPLC/UV analyses of AFD12 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 231 kb)
: Figure S15. HPLC/UV analyses of AFD14 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 242 kb)
: Figure S17. HPLC/UV analyses of AFD26 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% TFA; solvent B: acetonitrile; flow rate: 1.0 mL min− 1; 0–30 min, 95–0% A (linear gradient); 30–35 min 0% A; 35–36 min 0–95% A (linear gradient); 36–40 min 95% A. (PDF 233 kb)
Figure S4. HPLC/MS analyses of AFD2 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight). (PDF 313 kb)
Figure S19. Chemical structures of the suggested Streptomyces metabolites based on HPLC-UV/Vis and LC-MS analyses and by searching in AntiBase 2017. (PDF 251 kb)
Figure S6. HPLC/MS analyses of AFD4 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight). (PDF 421 kb)
: Figure S8. HPLC/MS analyses of AFD7 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight). (PDF 409 kb)
Figure S10. HPLC/MS analyses of AFD8 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight) (PDF 385 kb)
Figure S12. HPLC/MS analyses of AFD10 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight) (PDF 434 kb)
Figure S20. Chemical structures of the suggested Streptomyces metabolites based on HPLC-UV/Vis and LC-MS analyses and by searching in AntiBase 2017. (PDF 400 kb)
: Figure S14. HPLC/MS analyses of AFD12 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight) (PDF 431 kb)
: Figure S16. HPLC/MS analyses of AFD14 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. No clear mass was detected for this extract, howver it is very intersting based on the HPLC-UV/Vis profile (Fig. S17). (PDF 334 kb)
Figure S18. HPLC/MS analyses of AFD26 crude extract. HPLC-conditions: Detection wavelength 254 nm; solvent A: H2O/0.1% Formic acid, solvent B: CH3CN/0.1% Formic acid; flow rate: 0.5 mL min− 1; 0–4 min, 10% B; 4–22 min, 10–100% B; 22–27 min, 100% B; 27–29 min, 100–10% B; 29–30 min, 10% B. (MW = Molecular Weight). (PDF 410 kb)