Fig. 3

Hierarchical clustering (HC) and principal component analysis (PCA) of the qPCR assays. The data used in the Figs. 1 and 2 were combined and further analyzed with HC and PCA. a Heat-map based on HC. The samples and genes were differently colored based on the genus they belong. PCA score plot (b) and PCA loading plot (c) of the results are shown. For sample groups: Burkholderia,; Paraburkholderia,; Burkholderia + Paraburkholderia,; other, . For gene groups: Burkholderia sensu stricto-specific,; Paraburkholderia-specific,; Burkholderia sensu lato-conserved, . For sample names, ‘Mix-P’, ‘Mix-E’ and ‘Mix-E + P’ indicate mixture of two Burkholderia species, two Paraburkholderia species, and one Burkholderia and one Paraburkholderia species, respectively; ‘_exp’ and ‘_det’ indicate expected and detected values; the numbers after ‘Mix-E + P’ indicate the percentage of B. cenocepacia LMG 16656 in the mixtures. All three replicates of qPCR are shown